生态学报2017，Vol.37Issue(23)：8015-8028,14.DOI:10.5846/stxb201704270770
基于EPG技术分析茶树品种对茶小绿叶蝉的抗性及其相关的抗性物质
Resistance of tea cultivars to the tea green leafhopper analyzed by EPG technique and their resistance-related substances
摘要
Abstract
To thoroughly investigate probing behavior of the tea green leafhopper,Empoasca vitis (G~the),on tea plants,determine adequate electrical penetration graph (EPG) parameters as reliable indices for measuring tea plant resistance/ susceptibility levels to the leafhopper,and further analyze their potential correlations to the major defensive components in tea leaves,11 national or regional elite/specificity cultivars,such as Zijuan,Ningbohuangjinya and Huangguangyin,were studied using EPG techniques.Various leafhopper probing behaviors and EPG parameters were analyzed to evaluate the resistance/susceptibility of every cultivar to the leafhopper.Major (primary) tea leaf chemical components were determined using high-pressure liquid chromatography.Our results showed that:1) seven distinct EPG waveforms (Np,A,S,C,E,F,and R) with possible biological significance during leafhopper feeding were recorded,and each waveform and its regular emergence sequence were analyzed.The waveforms E and F,plus the waveform R (with unknown biological significance)were considered to be the resistance-related waveforms.2) During the 5-h testing period,the numbers of probing instances by the leafhopper on cultivars Huangguanyin and Longjing 43 were the highest,followed by those on cultivars Zijuan,Ningbohuangjinya and Jinguanyin,and the numbers on cultivars Tieguanyin and Anjibaicha were the lowest.The average durations of waveform E on cultivars Tieguanyin and Huangmudan were the longest,followed by the durations on cultivars Wuniuzao and Anjibaicha,and that on cultivar Zijuan was the shortest.The average duration of waveform F on cultivar Huangguanyin was the longest,followed by the durations on cultivars Zijuan,Longjing 43,and Ningbohuangjinya,and that on cultivar Jinguanyin was the shortest.3) These 11 tested tea cultivars were classified into two groups via cluster analysis based on the number of probing instances,and durations of waveforms E and F as indices;group Ⅰ:cultivars Anjibaicha,Wuniuzao,Zimudan,Zhongcha 108,Huangmudan,and Tieguanyin and group Ⅱ:cultivars Zijuan,Longjing 43,Ningbohuangjinya,Huangguanyin,and Jinguanyin.The resistance level of tea cultivars in group Ⅱ was stronger than that in group Ⅰ.4) From susceptible to resistant cultivars,the total catechin content rose,and it was negatively correlated with the duration of waveform E (P ＜ 0.05);however,the contents of caffeine,amino acids and soluble sugars were not significantly correlated with the duration of waveform E or F.It is suggested that the number of probing instances,and durations of waveforms E and F are relatively reliable EPG indices for measuring tea plant resistance/susceptibility levels to the leafhopper.Waveform R might be correlated with feeding-resistance in phloem,but needs further investigation.Catechins seemed to be the key defensive compounds in tea leaves against the leafhopper feeding.This study provides indepth reference information for further investigations on the resistance mechanisms of tea plants against pests.关键词
假眼小绿叶蝉/茶树/取食行为/EPG/抗虫性Key words
Empoasca vitis (G(o)the)/tea plant/probing behavior/electrical penetration graph/resistance against pest引用本文复制引用
郑雨婷,王梦馨,崔林,韩善捷,俞鹏飞,韩宝瑜..基于EPG技术分析茶树品种对茶小绿叶蝉的抗性及其相关的抗性物质[J].生态学报,2017,37(23):8015-8028,14.基金项目
浙江省自然科学基金项目(LY17C140002) （LY17C140002）
浙江省大学生科技创新项目(2016R40915,2017R409055) （2016R40915,2017R409055）
