土壤2017，Vol.49Issue(6)：1159-1165,7.DOI:10.13758/j.cnki.tr.2017.06.014
滩涂土壤固氮菌群落与环境因子的典范对应分析
Canonical Correspondence Analysis of Relationship Between Characteristics of Nitrogen-fixing Microbes Community and Environmental Factors in Mudflat Soil
摘要
Abstract
In order to study the characteristics of soil nitrogen-fixing microbial communities, an experiment of rice-astragalus smicus rotation was conducted for 4 consecutive years in mudflat soil,nifH gene abundance and community diversity in different depth of profile (0-10, 10-20, 20-40 and 40-80 cm) were investigated by PCR-DGGE, and canonical correspondence analysis (CCA) was used to study the correlation between nitrogen-fixing microbes and environmental factors. The results showed that rice-astragalus smicus rotation increased the richness and diversity indexes of nitrogen-fixing microbes in mudflat soil. The abundance and diversity indexes of nitrogen-fixing microbes were the highest in 10-20 cm soil under rice-astragalus smicusrotation and was the lowest in 40-80 cm soil under rice-fallow rotation, with Shannon-Weaver diversity indexes of 3.18 and 1.51, respectively. High correlations were observed between soil total nitrogen and ammonium nitrogen, EC and available P, pH and available K. CCA ordination analysis indicated that soil organic matter, ammonium nitrogen and available phosphorus were the main factors affecting nitrogen-fixing microbial communities in mudflat soil.关键词
滩涂盐碱地/nifH基因/PCR-DGGE/典范对应分析Key words
Mudflat soil/nifH Gene/PCR-DGGE/Canonical correspondence analysis分类
农业科技引用本文复制引用
蔡树美,徐四新,张翰林,张德闪,吕卫光,顾富家,诸海焘..滩涂土壤固氮菌群落与环境因子的典范对应分析[J].土壤,2017,49(6):1159-1165,7.基金项目
科技部星火计划(2015GA680001),上海市农业科学院卓越团队建设计划(农科创2017A-03),上海市科委成果转化项目(153919N0402),崇明县科技攻关项目(CKS2016-02)和上海市科技兴农推广项目(沪农科推字2015第2-2号)资助. （2015GA680001）
