物理化学学报2018，Vol.34Issue(1)：73-80,8.DOI:10.3866/PKU.WHXB201707043
不同条件下离体大鼠肝脏线粒体能量代谢微量热分析
Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions
摘要
Abstract
Isolated rat liver mitochondria were proposed as a model to monitor real-time heat metabolism. A high-throughput and sensitive thermal activity monitor III (TAM III) was used to detect the P-t curves of mitochondria under different conditions, including different mitochondrial concentrations, different substrates, different buffers,respiratory inhibitors, Ca2+, and CsA. We determined the thermokinetic parameters through calculation. The results showed that: (1) higher concentration of mitochondria led to faster energetic metabolism; (2) when succinate was the direct respiratory substrate, it promoted mitochondrial metabolism, in contrast to the condition when an indirect substrate,pyruvate, was used; (3) high concentration of Ca2+ (2.5 mmol·L-1) stimulated mitochondrial metabolism, however CsA, an inhibitor of mitochondrial permeability transition pores, could not reverse the Ca2+-induced mitochondrial alteration; (4)mitochondria in various buffers displayed different rates of heat metabolism, because of the different composition of the buffers; (5) mitochondrial metabolism was inhibited by respiratory inhibitors, especially NaN3, which is an inhibitor of Complex IV and which completely stopped the mitochondrial heat release.关键词
线粒体/微量热/代谢速率/呼吸链抑制剂/线粒体渗透转换Key words
Mitochondria/Microcalorimetry/Metabolic rate/Respiratory inhibitor/Mitochondrial permeability transition分类
化学化工引用本文复制引用
袁莲,刘玉娇,何欢,蒋风雷,李会荣,刘义..不同条件下离体大鼠肝脏线粒体能量代谢微量热分析[J].物理化学学报,2018,34(1):73-80,8.基金项目
The project was supported by the National Natural Science Foundation of China (21673166) and Natural Science Foundation of Hubei Province, China (2015CFC892).国家自然科学基金(21673166)和湖北省自然科学基金项目(2015CFC892)资助 （21673166）
