物理化学学报2018，Vol.34Issue(1)：99-107,9.DOI:10.3866/PKU.WHXB201706262
功能化离子液体修饰多壁碳纳米管固定化Candida antarctic lipase B
Study of Surface Modifications of Multiwalled Carbon Nanotubes by Functionalized Ionic Liquid to Immobilize Candida antarctic lipase B
摘要
Abstract
Multiwalled carbon nanotubes modified on the surface by imidazole ionic liquids with different types of anions (Br-, BF4-, PF6- or H2PO4-) as a new-generation carrier were fabricated. Subsequently, Candida antarctic lipase B (CALB)was immobilized on the functionalized MWNTs via physical attachment, and its enzymology properties were tested. The morphology of the MWNTs before and after the modification was determined by transmission electron microscopy (TEM),Raman spectroscopy, thermo gravimetric analysis (TGA), and X-ray photoelectron spectroscopy (XPS). These characterizations were used to study the influence of surface modification of materials on the enzymatic properties. The results revealed that CALB immobilized on MWNTs modified by ionic liquids not only had a higher specific activity as compared with that of CALB immobilized on pure MWNTs, but also better tolerance (high temperature, high pH),thermostability, and reusability. The different anions of ionic liquids, which were used to modify MWNTs, exert a remarkable effect on the properties of immobilized enzyme. The specific activity of MWNTs-IL (PF6-)-CALB were thehighest and were five times higher than that of MWNTs-CALB with no modification. The analysis of kinetic parameters of immobilized enzymes showed that the modification of ionic liquids for carriers enhanced the affinity between the enzyme and the substrate, so as to increase the enzyme activity.关键词
碳纳米管/固定化酶/离子液体/碳基材料/表面修饰Key words
Carbon nanotube/Immobilized enzyme/Ionic liquid/Carbon-based material/Surface modification分类
化学化工引用本文复制引用
相欣然,万晓梅,索红波,胡燚..功能化离子液体修饰多壁碳纳米管固定化Candida antarctic lipase B[J].物理化学学报,2018,34(1):99-107,9.基金项目
The project was supported by the National Natural Science Foundation of China (21676143), Qing Lan Project of Jiangsu Province and Program for Innovative Research Team in University of Jiangsu Province, China.国家自然科学基金(21676143), 青蓝工程和江苏高等学校优秀科技创新团队计划资助 （21676143）
