现代妇产科进展2018，Vol.27Issue(1)：9-13,5.DOI:10.13283/j.cnki.xdfckjz.2018.01.003
视黄醇代谢相关基因LRAT和RDH12在宫颈鳞癌细胞系中的抗肿瘤作用
The effect of retinol metabolism related genes LRAT/RDH12 in the progress of squa-mous cervical cancer cell line
摘要
Abstract
Objective:To investigate the effects of metabolism-related genes LRAT and RDH12 in cervical squamous cell carcinoma cell lines SiHa and CaSKi on cell proliferation,ap-optosis,cell invasion and migration. Methods:LRAT/RDH12 over expression plasmids were carried to SiHa and CaSKi cell lines,respectively. CCK-8 method was used to detect cell prolif-eration,further through the Annexin V-FITC ( green fluorescence) and propyl iodide organism ( PI) ( red fluorescence) dyeing transfection cells to detect the cell cycle and cell apoptosis. Tr-answell chamber was used to detect the migration and invation of cervical squamous carcinoma. Results:We successfully constructed LRAT and RDH12 expression vectors and transfected cells. In the G0/G1 phase SiHa and CaSki cell lines accounted for a higher proportion than the control group,and in the S phase the proportion was lower. Compared with the control group,the percentage of apoptotic cells in SiHa cell lines increased by about 11％ and 10％,respectively, and in CaSki cell lines,both groups were increased by about 7％. In the SiHa and CaSki cells, group transfected with LRAT and RDH12 , the number of the migration and invasion was less than the blank plasmid transfected group. Conclusions:By constructing LRAT and RDH12 ex-pression vector and successfully transfection cells,it was suggested that these two genes had in-hibitory effects on proliferation,apoptosis,and tumor invasion and migration in cervical cancer cell lines. It is suggested that this gene may be the tumor suppressor gene of cervical cancer.关键词
宫颈癌/视黄醇相关基因/LRAT/RDH12Key words
Cervical cancer/Retinol related genes/LRAT/RDH12分类
医药卫生引用本文复制引用
刘鹏飞,陈纳泽,向阳..视黄醇代谢相关基因LRAT和RDH12在宫颈鳞癌细胞系中的抗肿瘤作用[J].现代妇产科进展,2018,27(1):9-13,5.基金项目
国家自然科学基金资助项目(No:81472446) （No:81472446）
