现代妇产科进展2018，Vol.27Issue(1)：14-17,4.DOI:10.13283/j.cnki.xdfckjz.2018.01.004
NAC-1通过自噬介导卵巢癌细胞顺铂耐药的研究
The study of NAC-1 mediated cisplatin resistance in ovarian cancer cells by autophagy
摘要
Abstract
Objective:To investigate the role of NAC-1 in autophagy mediated cisplatin resistance in ovarian cancer cells. Methods:Autophagy and apoptosis of cisplatin-induced che-moresistant ovarian cancer cells ( SKOV3/DDP) were detected by Western blot and Flow cy-tometry. The expression of NAC-1 protein was detected. The effect of NAC-1 inhibition by siR-NA on autophagy,apoptosis and resistance of SKOV3/DDP cells were detected. Results:Cispla-tin could lead to increased autophagy and apoptosis of SKOV3/DDP,while NAC-1 protein ex-pression was increased. Downregulation of NAC-1 by siRNA could decrease autophagy and in-crease apoptosis in SKOV3/DDP cells treated with cisplatin. Meanwhile,cisplatin resistance was decreased. Conclusion:NAC-1 gene may participate in cisplatin resistance of ovarian cancer cells by regulating autophagy. Reducing the expression of NAC-1 gene may be used to improve the cytotoxicity of cisplatin on ovarian cancer cells.关键词
NAC-1/自噬/卵巢癌细胞/顺铂耐药Key words
NAC-1/Autophagy/Ovarian cancer cells/Cisplatin resistance分类
医药卫生引用本文复制引用
李冬冬,钟洁,王莉,杨雪娇,凌晨祁,梁延平..NAC-1通过自噬介导卵巢癌细胞顺铂耐药的研究[J].现代妇产科进展,2018,27(1):14-17,4.基金项目
杨浦区肿瘤预防专项行动计划与上海市东医院院级课题联合资助(No:2016YKT010) （No:2016YKT010）
