畜牧兽医学报2017，Vol.48Issue(12)：2301-2313,13.DOI:10.11843/j.issn.0366-6964.2017.12.010
基于转录组数据的蒙古牛皮肤组织抗寒相关信号通路及候选基因的筛选
Screening of Cold Tolerance-Related Signaling Pathways and Candidate Genes in Mongolia Cattle Skin Tissues Based on Transcriptome Data
摘要
Abstract
The aim of this study was to find out signaling pathways and candidate genes that related to cold tolerance of Mongolia cattle by RNA-sequencing and bioinformatics analysis of Mongolia cattle skin tissues in winter and summer.Transcriptome sequencing skin tissues of adult Mongolia cattle in winter and summer were carried out by Ion ProtonTM Sequencer,respectively.And then data processing,reference genome alignment,gene differential expression analysis,GO and KEGG enrichment analysis and candidate gene screening were applied on the transcriptome data;Additionally,the reliability of the transcriptome data was detected by Real-time PCR.The results showed that 86 954 060 and 69 837 009 reads were obtained from winter and summer groups,respectively;The percentage of the uniquely mapped reads was 73.21％ in winter group,and 75.55％ in summer group.There were 182 DEGs between winter and summer groups,compared to summer group,117 were up-regulated and 65 were down-regulated in winter group;Real-time PCR analysis showed that the transcriptome sequencing results were reliable;The results of GO analysis showed there were 21,39 and 224 terms enriched in the cellular components,molecular functions and biological processes,respectively;KEGG analysis showed that the DEGs were significantly enriched in lipid metabolism,blood coagulation,tyrosine metabolism,steroid hormone biosynthesis and retinol metabolism related pathways;8 candidate genes were screened out.In summary,lipid metabolism,blood coagulation and melanin synthesis related pathways and genes as well as hair synthesis related genes may play an important role in cold resistance of Mongolian cattle.关键词
蒙古牛/皮肤组织/转录组/信号通路/差异表达基因Key words
Mongolia cattle/skin tissue/transcriptome/signal pathway/differentially expressed genes分类
农业科技引用本文复制引用
齐昱,邢燕平,潘静,凌宇,曹宇,周欢敏..基于转录组数据的蒙古牛皮肤组织抗寒相关信号通路及候选基因的筛选[J].畜牧兽医学报,2017,48(12):2301-2313,13.基金项目
国家科技支撑计划项目(2011BAD18B00) （2011BAD18B00）
内蒙古自治区科技计划项目(20131403) （20131403）
