畜牧兽医学报2017，Vol.48Issue(12)：2323-2336,14.DOI:10.11843/j.issn.0366-6964.2017.12.012
氨基酸平衡低蛋白质日粮对育肥猪肠道黏膜抗菌肽与微生物区系的影响
The Effect of the Low Protein Level Diets with Balanced Amino Acids on Intestinal Mucosa Antimicrobial Peptide and Microbial Flora of Fattening Pigs
摘要
Abstract
This research was carried out to study the effect of low protein level diets with balanced amino acids on intestinal antimicrobial peptide and microbial flora in fattening pigs.Twenty pigs (Duroc× Landrace × Yorkshire) weighed (60 ± 2.5) kg were randomly assigned into 4 treatments:14.0％ protein level diet (14.0％ CP),12.5％ protein level diet supplemented with Glu,Lys,Met,Thr,Trp (12.5％CP),11.0％ protein level diet supplemented with Glu,Lys,Met,Thr,Trp (11.0％ CP),11.0％ protein level diet supplemented with Glu,Lys,Met,Thr,Trp and BCAA(Val、Ile、Leu)(11.0％ CP +BCAA).Each treatment had 5 replicates,each replicate had one pig.The trial was divided into 5-d pre-trial period and 30-d test period.The results showed as follows:(1) Reducing the dietary protein level led to lower concentration of urea nitrogen and uric acid(P＜0.05);(2) Low protein level diets significantly reduced the level of β-defensin-2 in jejunum and ileum mucosa as well as mRNA expression levels of ANG1 and ANG4 (P＜0.05).(3) Different protein levels of diets had significant effect on the partial amino acid compositions of the ileum and rectum microorganisms (P＜0.05);(4)The Observed species of 12.5％ CP group were increased significantly compared with 14.0％ CP group (P＜0.05).The Shannon,PD of 11.0％ CP group and Shannon of 11.0％ CP+BCAA group were decreased significantly (P＜0.05) compared with 14.0％ CP group.There was no significant difference in Bacteroidetes and Firmicutes abundance among 4 groups (P＞0.05).The relative abundance of Acidobacteria and Gemmatimonadetes in 12.5％ CP group and 11.0％ CP group as well as the relative abundance of Gemmatimonadetes in 11.0％ CP+BCAA group were decreased significantly compared with 14.0％ CP group(P＜0.05).The relative abundance of Proteobacteria in 14.0％ CP group was higher than that in 12.5％ CP group and 11.0％ CP+BCAA group (P＜0.05) and was not significantly different with 11.0％ CP group (P ＞ 0.05),the relative abundance of Campylobacteraceae of 14.0％ CP group was higher than that in other groups(P＜0.05).The relative abundance of Coprococcus and Clostredium_sensu_stricto in 14.0％ CP group were higher than that in other groups (P＜0.05).The relative abundance of Lachnospiraceae FCS020 in 11.0％ CP group and 11.0％ CP±BCAA group were higher than that in 12.5％ CP group and 14.0％ CP group (P＜0.05).Low protein level diets with balanced amino acids can improve nitrogen utilization and maintain the balance of the intestinal microbial flora.Low protein balanced diets with BCAA help to increase the amino acid balance and maintain intestinal immune function.关键词
低蛋白质日粮/微生物区系/菌体氨基酸/抗菌肽Key words
low protein level diet/microbial flora/amino acids of mycelium/antimicrobial peptides分类
农业科技引用本文复制引用
王瑶,孙志洪,许庆庆,刘金艳,李金龙,唐志如..氨基酸平衡低蛋白质日粮对育肥猪肠道黏膜抗菌肽与微生物区系的影响[J].畜牧兽医学报,2017,48(12):2323-2336,14.基金项目
国家973重点基础研究发展计划项目(2013CB127303) （2013CB127303）
重庆市自然科学基金(cstc2016jcyjA1414) （cstc2016jcyjA1414）
国家948项目(2015274) （2015274）
西南大学博士基金(SWU111002) （SWU111002）
西南大学基本科研业务费(XDJK2017D037) （XDJK2017D037）
国家留学基金委西部计划(201508505170) （201508505170）
