畜牧兽医学报2017，Vol.48Issue(12)：2347-2357,11.DOI:10.11843/j.issn.0366-6964.2017.12.014
不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响
Effects of Diets with Different NFC/NDF Levels on the Rumen Fermentation Parameters and Bacterial Community in Male Calves
摘要
Abstract
This study assessed the effects of diets with different non-fiber carbohydrate (NFC)/neutral detergent fiber (NDF) levels on rumen fermentation parameters and bacterial community of male calves.Thirty 2-3 month-old healthy weaned male calves with (94.38 ± 0.25)kg were randomly divided into 2 groups with 15 calves each,and were fed 2 types of total mixed ratio diet with the same crude protein level and different NFC/NDF ratios (1.35 (A) and 0.80 (D)),respectively.The experiment lasted for 105 d,with 15 d of adaptation period and 90 d of formal period.Six calves in each group were selected to collect rumen fluid through oral for 2 hours after morning feeding on the 120,150 and 180 d.The pH,volatile fatty acids (VFA) and NH3-N concentration and rumen microbial diversity of the rumen fluid samples were determined.The results showed that:1) The dietary NFC/NDF levels had no significant effect on rumen fluid pH and total VFA concentration (P＞0.05);2) Acetate,NH3-N concentration and acetate/propionate ratio in group A were significantly lower than that in group D (P＜0.05),propionate content in group A were significantly higher than that in group D (P＜0.05);3) The microbial diversity parameters of rumen fluid of calves in group A were significantly lower than that in group D (P＜0.05);4) At the phylum level,bacteriodetes proportion accounting for the total number of sequences of calves in group A was significantly higher than that in group D (P＜0.05),Christensenellaceae_R-7 and Prevotellaceae_UCG-003 proportions accounting for the total number of sequences of calves in group D was significantly higher at the genus level than that in group A (P＜0.05);5) With the age increasing,the diversity indices except for Shannon index,were significantly different between the 2 groups (P＜0.05),but there was no significant difference in rumen microbial composition between the 2 groups(P＞0.05).In summary,the dietary NFC/NDF levels significantly affect the species and abundance of the rumen microorganisms,and the level of genus of microorganisms,finally affect the yield of acetate and propionate.关键词
断奶肉犊牛/非纤维性碳水化合物/中性洗涤纤维/瘤胃发酵参数/瘤胃微生物区系Key words
weaned beef calves/non-fiber carbohydrate/neutral detergent fiber ratio/rumen fermentation parameters/rumen microbial community分类
农业科技引用本文复制引用
李岚捷,成述儒,刁其玉,付彤,毕研亮,王安思,李明,屠焰..不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响[J].畜牧兽医学报,2017,48(12):2347-2357,11.基金项目
公益性行业科技项目(201303143) （201303143）
河南省科技开放合作项目(152106000029) （152106000029）
河南省现代农业产业技术体系建设专项资金(Z-2013-08-01) （Z-2013-08-01）
