畜牧兽医学报2017，Vol.48Issue(12)：2358-2364,7.DOI:10.11843/j.issn.0366-6964.2017.12.015
经密码子优化的猪Ficolin α在昆虫细胞的表达及其体外抗病毒作用初步分析
Expression of Codon-optimized Porcine Ficolin α in Insect Cell and Its Antiviral Activity in vitro
摘要
Abstract
To get native porcine Ficolin α,Bac-to-Bac baculovirus expression system was used.Firstly,rBacmid-Ficolin α was constructed,and then transfected into sf9 insect cells to obtain recombinant baculovirus rBV-Ficolin α.The optimized expression conditions were detected by Western blot and analyzed by gray level using Image J software.Followed by purification,antiviral activity of target protein was assessed in vitro.Western blot results showed that the expression level of Ficolin α in transfected sf 9 insect cells was higher at the infection (MOI) of 7.5 when they were harvested at 96 h,and porcine Ficolin α had the antivirus activity against porcine reproductive and respiratory syndrome virus (PRRSV).In conclusion,porcine Ficolin α was successfully expressed in sf9 insect cells and showed strongly anti-PRRSV activity.This study provided a material basis for the study of antiviral activity and its mechanism of porcine Ficolin α.关键词
猪Ficolin/杆状病毒-昆虫细胞表达系统/抗病毒作用Key words
porcine Ficolin/baculovirus-insect cell system/antiviral activity分类
农业科技引用本文复制引用
李兰,乔绪稳,张元鹏,陈瑾,郑其升,侯继波..经密码子优化的猪Ficolin α在昆虫细胞的表达及其体外抗病毒作用初步分析[J].畜牧兽医学报,2017,48(12):2358-2364,7.基金项目
农业部公益性行业(农业)科研专项经费项目(201303046) （农业）
