畜牧兽医学报2017，Vol.48Issue(12)：2407-2413,7.DOI:10.11843/j.issn.0366-6964.2017.12.021
不同年龄牦牛肾的组织学特征
Histological Study on Age-Associated Changes in the Kidney of the Yak
摘要
Abstract
This experiment was conducted to investigate the histological characteristics and age-related changes in yak kidney.Newborn (1-7 days old,n=5),adult (3-6 years old,n=5) and old (7-10 years old,n=5) yaks were selected as the subjects,and histological and statistical methods were used to observe the changes in yak kidney.The relevant data of kidney units was analyzed and measured by the image analysis software.Histological observations showed that yak kidneys consisted of the surface of the membrane and the internal substance.The malpighian corpuscles were composed of glomeruli and renal capsules.The wall of the renal capsule was rich in collagen fibers and the basal membrane of malpighian corpuscles was PAS positive.The kidney tubules included proximal tubules and distal tubules,which are rich in collagen fibers and had a positive PAS on the epithelium basal membrane.The results of measuring showed that the number of glomeruli per unit field (2.3 × 106 μm2) decreased with age;while the number of glomerular cells and the size of glomerular nuclei increased with age;the differences was in the maximum crosssectional area of glomeruli and malpighian corpuscle were the largest in adult,followed by the differences in the aged,with those in the newborn being the smallest.In addition,with the age increasing,the trend of changes in the number of tubule epithelial cells in the kidney cortex and medulla per unit field (2.3× 104 μm2),the size of proximal tubule and distal tubule epithelial nuclei were the same as the number of glomeruli and cells in glomeruli,the size of glomeruli nuclei,and the maximum cross-sectional area changes of glomeruli and malpighian corpuscle.The results suggested that the histological structures of yak kidney changed with the increase of age.Moreover,it is presumed that the contributing factors to this change might include glomeruli sclerosis and reduction of substantial cells,however,further empirical investigation is required to be conclusive.关键词
牦牛/肾小球/肾小管上皮细胞/增龄性变化Key words
yak/glomeruli/kidney tubular epithelial cells/age-associated changes分类
农业科技引用本文复制引用
王婷,崔燕,何俊峰,刘鹏刚,张倩,杨雪..不同年龄牦牛肾的组织学特征[J].畜牧兽医学报,2017,48(12):2407-2413,7.基金项目
国家自然科学基金(31360594 （）
31572478) （）
