应用生态学报2017，Vol.28Issue(12)：3881-3890,10.DOI:10.13287/j.1001-9332.201712.040
退化高寒草甸土壤微生物及酶活性特征
Characteristics of soil microbes and enzyme activities in different degraded alpine meadows
摘要
Abstract
Soil microbial biomass C and N,microbial diversities and enzyme activity in 0-10 cm and 10-20 cm soil layers of different degraded grasslands (non-degradation,ND;light degradation,LD;moderate degradation,MD;sever degradation,SD;and black soil beach,ED) were measured by Biolog and other methods.The results showed that:1) There were significant differences between 0-10 cm and 10-20 cm soil layers in soil microbial biomass,diversities and invertase activities in all grasslands.2) The ratio of soil microbial biomass C to N decreased significantly with the grassland degradation.In the 0-10 cm soil layer,microbial biomass C and N in ND and LD were significantly higher than that in MD,SD and ED.Among the latter three kinds of grasslands,there was no difference for microbial biomass C,but microbial biomass N was lower in MD than in the other grasslands.The average color change rate (AWCD) and McIntosh Index (U) also decreased with grassland degradation,but only the reduction from ND to MD was significant.There were no differences among all grasslands for Shannon index (H) and Simpson Index (D).The urease activity was highest in MD and SD,and the activity of phosphatase and invertase was lowest in ED.In the 10-20 cm soil layer,microbial biomass C in ND and LD were significantly higher than that in the other grasslands.Microbial biomass N in LD and ED were significantly higher than that in the other grasslands.Carbon metabolism index in MD was significantly lower than that in LD and SD.AWCD and U index in ND and LD were significantly higher than that in ED.H index and D index showed no difference among different grasslands.The urease activity in ND and MD was significantly higher than that in the other grasslands.The phosphatase activity was highest in MD,and the invertase activity was lowest in MD.3) The belowground biomass was significantly positively correlated with microbial biomass,carbon metabolic index and phosphatase activity,and the urease activity was negatively correlated with microbial biomass N,H index and D index.关键词
高寒草甸/退化/土壤微生物量碳氮/碳源代谢/酶Key words
alpine meadow/degradation/soil microbial biomass C and N/carbon metabolic index/enzymes引用本文复制引用
尹亚丽,王玉琴,鲍根生,王宏生,李世雄,宋梅玲,邵宝莲,温玉存..退化高寒草甸土壤微生物及酶活性特征[J].应用生态学报,2017,28(12):3881-3890,10.基金项目
本文由国家自然科学基金项目(31560660)、青海省科技项目(2016-ZJ-729)和青海大学中青年科研基金项目(2015-QNY-5)资助 This work was supported by the National Natural Science Foundation of China (31560660),the Science and Technology Project of Qinghai Province,China (2016-ZJ-729) and the Scientific Research Foundation for Young and Middle-aged of Qinghai University (2015-QNY-5) （31560660）
