农学学报2017，Vol.7Issue(12)：18-21,27,5.
异源表达细菌β-甘露聚糖酶研究进展
Heterologous Expression Bacterialβ-mannanase:Research Progress
摘要
Abstract
Mannanase is a key enzyme for hydrolyzing mannan, and is widely found in plants, animals and microorganisms, particularly in bacteria. In order to meet the needs of industrial production, heterologous expression of bacterial-derived mannanase gene is studied in order to obtain high-quality mannanase. In this paper, the origin and enzymatic properties of mannanase were reviewed, and the heterologous expression of bacterial mannanase was summarized concerning the aspects of improving the enzyme activity, expanding the pH range and improving the safety, including heterologous expression of Escherichia coli, Bacillus subtilis and Pichia pastoris. The use of microbial resources can broaden the application of mannanase, and the use of microbial technology can improve the amount of enzyme production to meet industrial needs, these lay a foundation for the determinate evolution and transformation of mannanase.关键词
细菌/甘露聚糖酶/分子生物学/异源表达/应用Key words
Bacteria/β-mannanase/Molecular Biology/Heterologous Expression/Application分类
轻工纺织引用本文复制引用
那金,王瑶,郭尚旭,赵丹..异源表达细菌β-甘露聚糖酶研究进展[J].农学学报,2017,7(12):18-21,27,5.基金项目
哈尔滨市科技局青年后备人才项目"功能性乳酸菌发酵酸菜生态学过程研究"(2014RFQXJ101) （2014RFQXJ101）
黑龙江省政府博士后项目"疣孢漆斑菌(Myrothecium verrucaria)NF-05漆酶的产生、特性及应用研究"(LBH-Z15214). （Myrothecium verrucaria）
