中国生物防治学报2017，Vol.33Issue(6)：725-731,7.DOI:10.16409/j.cnki.2095-039x.2017.06.002
两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较
Comparison of Susceptibilities to Cry1Ac between Two Populations of Plutella xylostella with Different Resistance Backgrounds to Chemical Insecticides
摘要
Abstract
For rational use of chemical insecticides and bio-pesticides Bt in the control of Plutella xylostella,the relationship between the resistance of P.xylostella to chemical insecticides and susceptibility to Bt Cry1Ac protein was investigated.The susceptibilities of two P.xylostella populations with different resistance backgrounds to 9 commonly used insecticides and Cry1Ac protein were determined using leaf-dipping method and the cross-resistance of the two P.xylostella populations to chemical insecticides and Cry1Ac protein was clarified.The results showed that the GDBL population,collected from fields in Boluo County,Guangdong province,exhibited different resistance levels to the nine commonly used insecticides,except to Cry1Ac protein.Though the laboratory population BJNK showed resistance to beta-cypermethrin,abamectin,indoxacarb,spinosadand chlorfenapyr,BJNK population is still susceptible to the other five insecticides including Cry1Ac protein.Our results showed that the two P.xylostella populations of different resistance backgrounds to several insecticides are still susceptible to Cry1Ac protein.No cross-resistance was observed between the resistance to chemical insecticides and that to Cry1Ac protein,because the resistance mechanisms to chemical insecticides are different from that to CrylAc protein.Therefore,Bt can be used for effectively control of field P.xylostella populations that have already exhibited resistance to a variety of chemical insecticides.关键词
小菜蛾/化学农药/生物农药/BtCry1Ac蛋白/抗药性Key words
Plutella xylostella/chemical insecticides/biological insecticide/Bt Cry1Ac protein/resistance分类
农业科技引用本文复制引用
许国升,王翠翠,张贤,束长龙,李振宇,史雪岩,张杰..两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较[J].中国生物防治学报,2017,33(6):725-731,7.基金项目
国家重点研发计划(2017YFD0201204) （2017YFD0201204）
