中国生物防治学报2017，Vol.33Issue(6)：817-825,9.DOI:10.16409/j.cnki.2095-039x.2017.06.015
产生ACC脱氨酶的PGPR种衣剂对黄瓜细菌性茎软腐病的防治效果
Bio-control of PGPR Seed Coating Producing ACC Deaminase to Cucumber Bacterial Stem Soft Rot Disease
摘要
Abstract
The plant growth promoting rhizo bacteria (PGPR) producing 1-amino cyclopro-pane-l-carboxylic acid (ACC) deaminase were isolated and identified from the rhizosphere soil of seashore halophytesin order to prevent and control cucumber bacterial stem soft rot,a new disease in winter greenhouse cucumber in recent years.7 species of PGPR including Stenotrophomonas maltophilia,Raoultella terrigena,Agrobacterium tumefaciens,Klebsiella oxytoca,Pantoea agglomerans,Enterobacter aerogenes and Kluyvera cryocrescens were identified.The activity of ACC deaminase in Stenotrophomonas maltophilia CRG-2 was the highest,324.3 μmol/(mg·h) in 22 strains.At the same time,the biocontrol effect of biological seed-coating by mixing isolate strain CRG-2 and Bacillus subtilis with additives to cucumber bacterial stem soft rot were tested in the field.The control effect of the seed coating agent formed by the mixture of strain CRG-2 and Bacillus subtilis to cucumber bacterial stem soft rot reached 73.13％ in the field,higher than zhongshengrnycin with control effect of 15.79％.The encouraging effect of seeding coat on biological control suggested that it had the potential to be applied widely in the cucumber bacterial stem soft rot control in the future.关键词
植物根际促生菌(PGPR)/嗜麦芽寡养单胞菌/ACC脱氨酶/黄瓜细菌性茎软腐病/防治效果Key words
plant growth promoting rhizobacteria (PGPR)/Stenotrophomonas maltophilia/ACC deaminase/cucumber bacterial stem soft rot/control effect分类
农业科技引用本文复制引用
贺字典,闫立英,石延霞,李宝聚,高玉峰,张明珠,孙健..产生ACC脱氨酶的PGPR种衣剂对黄瓜细菌性茎软腐病的防治效果[J].中国生物防治学报,2017,33(6):817-825,9.基金项目
河北省科技支撑农业关键共性技术攻关专项(17226914D) （17226914D）
秦皇岛市科技支撑计划项目(2012YB002) （2012YB002）
河北省现代农业产业技术体系蔬菜创新团队建设资助项目(QN2015101) （QN2015101）
