中国现代医学杂志2017，Vol.27Issue(30)：1-7,7.DOI:10.3969/j.issn.1005-8982.2017.30.001
DcR3基因对胰腺癌裸鼠移植瘤化疗敏感性的影响
Effect of Decoy receptor 3 on chemosensitivity in nude mice model of pancreatic cancer
摘要
Abstract
Objective To investigate the effect of Decoy receptor 3 (DcR3) on chemosensitivity of human pancreatic cancer cells and potential mechanisms.Methods Pancreatic cancer AsPC-1 cells in log-growth phase (1 ×106 cells) were subcutaneously injected in nude mice.Expression of DcR3 was blocked through small interfering ribonucleic acid (siRNA).Seven days post injection,tumor-bearing mice were then randomly divided into 4 groups (n =10 group):control group,chemotherapy group,sham siRNA + chemotherapy group and DcR3 siRNA + chemotherapy.Xenograft of human pancreatic cancer AsPC-1 cells was measured among the groups.Expression level of DcR3 was determined by ELISA and Western blot.Apoptosis rate was detected by TUNEL analysis.Expression levels of FasL protein,Caspase-8,and Caspase-3 were measured by Western blot and RT-PCR.Results Tumor weight (gram) in chemotherapy group,sham siRNA + chemotherapy group and DcR3 siRNA + chemotherapy group was significantly decreased when compared with control group (0.63 ± 0.04 vs 0.95 ±0.03,P=0.000,0.67±0.02 vs 0.95 ±0.03,P=0.000,0.17 ±0.06 vs 0.95 ±0.03,P=0.000),respectively.Silencing of DcR3 expression dramatically reduced weight of tumor when compared with sham siRNA + chemotherapy group (0.17 ± 0.06 vs 0.67 ± 0.02 vs 0.95 ± 0.03,P=0.000).Inhibitory rate of tumor growth in chemotherapy group,sham siRNA + chemotherapy group and DcR3 siRNA + chemotherapy group increased when compared with control group [(35.87 ± 4.58)％ vs (0.00 ± 0.00)％,P=0.000,(40.68 ± 4.16) ％ vs (0.00 ± 0.00) ％,P=0.000,(90.25 ± 2.53) ％ vs (0.00 ± 0.00) ％,P=0.000],respectively.Silencing of DcR3 expression dramatically increased inhibitory rate of tumor growth when compared with sham siRNA + chemotherapy group [(90.25 ± 2.53) ％ vs (0.67±0.02) ％ vs (40.68 ± 4.16) ％,P =0.000].Apoptosis rate in chemotherapy group,sham siRNA + chemotherapy group and DcR3 siRNA + chemotherapy group were significantly increased when compared with control group [(14.8 ± 2.65)％ vs (6.3 ± 2.21)％,P=0.000,(14.5 ± 3.06) ％ vs (6.3 ± 2.21) ％,P =0.000,(54.6 ± 3.23) ％ vs (6.3 ± 2.21) ％,P =0.000],respectively.Silencing of DcR3 expression dramatically reduced weight of tumor when compared with sham siRNA + chemotherapy group [(54.6± 3.23)％ vs (14.5 ± 3.06)％,P=0.000].The expression levels of FasL,Caspase-8,and Caspase-3 in DcR3 siRNA + chemotherapy group were downregulated when compared with the remaining 3 groups (P=0.000).Conclusion Silencing of DcR3 gene can increase the chemosensitivity of human pancreatic cancer by promoting FasL/Caspase-mediated cells apoptosis.关键词
胰腺癌/诱骗受体-3/RNA干扰/凋亡/化疗敏感性Key words
pancreatic cancer/DcR3/small interfering ribonucleic acid/apoptosis/chemosensitivity分类
医药卫生引用本文复制引用
肖华平,谢辉,罗春阳,李庆,方玉江..DcR3基因对胰腺癌裸鼠移植瘤化疗敏感性的影响[J].中国现代医学杂志,2017,27(30):1-7,7.基金项目
湖南省自然科学基金(No:14JJ3136) （No:14JJ3136）
郴州市科技局科研基金(No:CZ2013096) （No:CZ2013096）
