中国现代医学杂志2017，Vol.27Issue(30)：8-14,7.DOI:10.3969/j.issn.1005-8982.2017.30.002
新型骨软骨支架辅助Mosaicplasty技术和携带增强基因的ADSCs对大面积骨软骨缺损的修复效果
Effect of new osteochondral scaffold-assisted Mosaicplasty technology genetically enhanced ADSCs on repair of large area osteochondral defect
摘要
Abstract
Objective To investigate the effect of new osteochondral scaffold-assisted Mosaicplasty technology and genetically enhanced adipose-derived stem cells (ADSCs) on repair of large area osteochondral defect.Methods Beagle dog model of cartilage full-thickness defect was established.The defect was filled with multiple cartilage scaffolds through Mosaicplasty technology.No fixation was performed in group E.BMP-2-ADSCs was implanted in scaffold in group D.SF/CS/nHA was implanted in scaffold in group C.SF/CS/nHA combined with BMP-2-ADSCs was implanted in scaffold in group B.SF/CS/NHA and BMP-2-ADSCs combined with PRP gel complex was implanted in scaffold in group A.Morphological changes of tissue were observed at the 4th,8th,and 16th week post operation,and tissues were harvested at time point of the 16th week post operation.Collagen Ⅰ and Collagen Ⅱ mRNA were measured by PRC.Ultimate stress,strain value and the corresponding elastic modulus of the cartilage were determined.Results Compared with group E,cartilages in group A-D experienced better healing procedure with improvement of smooth surface.Group A witnessed dramatically improved cartilages when compared with group B-D (P＜ 0.05).The expression levels of collagen protein Ⅰ and collagen Ⅱ,ultimate stress,strain value and the corresponding elastic modulus were increased significantly in group A when compared with the remaining groups (P＜ 0.05).Conclusion New osteochondral scaffold filled with SF/CS/nHA,BMP-2-ADSCs and PRP gel complex through Mosaicplasty a technology can be therapeutic option for repair of large area cartilage defects.关键词
仿生支架/镶嵌移植术/骨软骨缺损/富血小板血浆/组织工程Key words
biomimetic scaffold/mosaic transplantation/osteochondral defect/platelet-rich plasma/tissue engineerin分类
医药卫生引用本文复制引用
阮世强,邓江,徐林,黄文良,田仁元..新型骨软骨支架辅助Mosaicplasty技术和携带增强基因的ADSCs对大面积骨软骨缺损的修复效果[J].中国现代医学杂志,2017,27(30):8-14,7.基金项目
贵州省科学技术项目(No:[2016]1420) （No:[2016]1420）
国家自然科学基金(No:81660367) （No:81660367）
