中国现代医学杂志2017，Vol.27Issue(30)：15-20,6.DOI:10.3969/j.issn.1005-8982.2017.30.003
棕榈酸对转化生长因子β1刺激的肝星状细胞增殖、凋亡及Caspase-12蛋白表达的影响
Effect of palmitic acid on caspase-12 and proliferation and apoptosis of hepatic stellate cells
摘要
Abstract
Objective To investigate the effect of palmitic acid (PA) on caspase-12 and proliferation and apoptosis in rat hepatic stellate cells (HSCs) when stimulated by transforming growth factor-β1 (TGF-β1).Methods HSCs were randomly divided into five groups:Sham group,TGF-β1 group,TGF-β1 + low PA group,TGF-β1 + middle PA group and TGF-β1 + high PA group.HSCs were stimulated by TGF-β1 (5 ng/ml) for 24 hours followed by co-incubation with PA under different doses (50,100,and 200 umol/L).No intervention was performed in Sham group.Cell proliferation was detected by MTT;morphological manifestations of cells were obtained by transmission electronmicroscope;apoptosis rate was determined by flow cytometry;expression levels of caspase-12 was measured by Immunohistochemistry.Results Cellular proliferation was significantly inhibited with PA treatment in a dose dependent manner (P ＜ 0.05).Transmission electronmicroscope showed typical apoptotic features such as small nuclei,large numbers of mitochondria vacuoles and obvious edge sets of DNA.Treatment of PA dramatically increased apoptosis rate when compared with TGF-β1 group [(7.26± 0.46)％ vs (1.70 ± 0.14) ％,P＜ 0.05;(11.24 ± 0.52) ％ vs (1.70 ± 0.14) ％,P＜ 0.05;(15.55 ± 0.31) ％ vs (1.70 ± 0.14) ％,P ＜ 0.05].Expression of caspase-12 protein increased significantly with PA in dose dependent manner (P ＜0.05).Conclusion PA inhibits cellular proliferation through promoting caspase-12 mediated apoptosis in HSCs.关键词
肝星状细胞/棕榈酸/细胞增殖/细胞凋亡/半胱氨酸天冬氨酸蛋白酶12(caspase-12)Key words
hepatic stellate cell/palmitic acid/proliferation/apoptosis/caspase-12分类
医药卫生引用本文复制引用
李雅楠,闫宇,王铭,刘芳,肖永红..棕榈酸对转化生长因子β1刺激的肝星状细胞增殖、凋亡及Caspase-12蛋白表达的影响[J].中国现代医学杂志,2017,27(30):15-20,6.基金项目
河北省自然科学基金资助项目(No:H2015209043) （No:H2015209043）
