中国现代医学杂志2017，Vol.27Issue(30)：21-25,5.DOI:10.3969/j.issn.1005-8982.2017.30.004
上调XB130表达对人肝癌HepG2细胞凋亡和增殖的影响
Effect of XB130 on human hepatocellular carcinoma HepG2 cells
陆林 1李东升 1白光1
作者信息
- 1. 锦州医科大学附属第一医院普外科,辽宁锦州121001
- 折叠
摘要
Abstract
Objective To investigate the effect of XB130 on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cell lines.Methods Western blot and qRT-PCR were utilized to determine expression of XB130 in hepatocellular carcinoma cells.Over expressed plasmid pCMV-EGFP-XB130 was transfected into HepG2 cells.Expression of XB130 was identified by Immunofluorescence,Western blot,and qRT-PCR.PI3K/Akt pathway related proteins were measured by Western blot;proliferation and apoptosis rate were measured by MTT assay and Flow cytometry,respectively.Results XB130 protein and mRNA were detected in different hepatocellular carcinoma cell-lines including Hep3B,Huh7,HepG2 and MHCC97H while HepG2 showed the lowest concentration of XB130 (P=0.001).p-p21,p-p27,p-FOXO3a and pro-Caspase 9 protein in pCMV-EGFP-XB130 group was up-regulated significantly when compared with EGFP control group (t=4.652,3.021,3.558 and 6.743,P＜0.05);XB130 overexpression increased cellular proliferation (P=0.001)while decreased apoptosis at the 72nd and 96th (P=0.001) in HepG2.Conclusion Up-regulation of XB130 increases proliferation while inhibits apoptosis via modulating PI3K/Akt signal pathway in hepatocellular carcinoma HepG2.关键词
XB130/肝细胞癌/增殖/凋亡Key words
XB130/hepatocellular carcinoma/proliferation/apoptosis分类
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陆林,李东升,白光..上调XB130表达对人肝癌HepG2细胞凋亡和增殖的影响[J].中国现代医学杂志,2017,27(30):21-25,5.
