中国畜牧兽医2017，Vol.44Issue(12)：3391-3400,10.DOI:10.16431/j.cnki.1671-7236.2017.12.001
北极狐及乌苏里貉抑制素α亚基基因克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Inhibin α-subunit Gene of Arctic Fox and Wusuli Raccoon Dog
摘要
Abstract
This study was aimed to clone the inhibin α(INHα) subunits gene of arctic fox and Wusuli raccoon dog and analyze its genetic structure with bioinformatics.According to the published sequence of INHα subunit gene (GenBank accession No.XM_545660.5),one pair of specific PCR primers were designed.The INHα subunit gene of arctic fox and Wusuli raccoon dog were amplified by RT-PCR.The target gene was linked into cloning vector to be sequenced and systemically analyzed by bioinformatics.The results showed that INHα subunit gene fragment of arctic fox and Wusuli raccoon dog included an 1 107 bp whole length CDS (encoding 369 amino acids).The INHα subunit gene of arctic fox and Wusuli raccoon dog shared 97.9％ and 97.6％ of similar nucleotide sequence with INHα subunit gene of dog,respectively.Phylogenetic analysis showed that the INHα subunit gene of arctic fox and Wusuli raccoon dog were genetically lose to dog,and it was highly conserved in different species and evolutionary processes.The prediction of INHα subunit tertiary structure showed that the cysteine knot directs members of the INHα subunit to adopt a "butterfly-shaped" or "open hand" configuration,with the α-helix defining the "wrist" and 3-sheets forming the "fingers" of the molecule.The INHα subunit gene of arctic fox and Wusuli raccoon dog were cloned successfully and analyzed by bioinformatics software,which would help to research the biological function of inhibin in the simultaneous development of oocyte-granulosa cells.关键词
北极狐/乌苏里貉/抑制素α(INHα)亚基基因/克隆/生物信息学分析Key words
arctic fox/Wusuli raccoon dog/INHα subunit gene/cloning/bioinformatics analysis分类
生物科学引用本文复制引用
张宇飞,魏海军,许保增,李晓霞,王士勇,曹新燕,刁云飞,杨镒峰,陈秀敏,赵伟刚,赵蒙..北极狐及乌苏里貉抑制素α亚基基因克隆及生物信息学分析[J].中国畜牧兽医,2017,44(12):3391-3400,10.基金项目
中国农业科学院创新工程(特种经济动物繁殖创新团队) （特种经济动物繁殖创新团队）
吉林省国际合作项目(20170414049GH) （20170414049GH）
国家自然基金面上项目(31772606) （31772606）
中国农业科学院基本科研业务费专项(1610342017025) （1610342017025）
