中国畜牧兽医2017，Vol.44Issue(12)：3446-3452,7.DOI:10.16431/j.cnki.1671-7236.2017.12.008
中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞中NF-κB、TNF-α、IL-6mRNA表达的影响
Effects of Compound Chinese Herbal Medicinal Polysaccharide Extraction on NF-κB,TNF-α and IL-6 mRNA Expression in Different MHC B-Lβ Ⅱ Genotype of Chickens Lymphocyte
摘要
Abstract
In order to explore the effect of compound Chinese herbal medicines polysaccharides (cCHMPS) on immunomodulatory in different MHC B-Lβ Ⅱ genotype chickens,200 White feather broiler were chosen and PCR-SSCP technique was applied to analyze the polymorphism of MHC B-Lβ Ⅱ gene.The peripheral blood were collected according to the different MHC B-Lβ Ⅱ genotype,and the lymphocytes were isolated from peripheral blood and the cCHMPS were added with a final concentration of 100,75,50 and 0 μg/mL for co-culturing 24 h.Then the expression of NF-κB,TNF-α,IL-6 mRNA in lymphocyte using Real-time PCR method were detected.The results showed that:Compared with the control group,different does of cCHMPS could significantly improve NF-κB,TNF-α,IL-6 mRNA expression levels in chicken with different MHC B-Lβ Ⅱ genotypes (P＜0.05),and when the cCHMPS concentration was 50 μg/mL,the NF-κB,TNF-α,IL-6 mRNA expression levels in lymphocyte of AB and AA genotype chicken were significantly higher than that of other groups (P＜0.05).The NF-κB,IL-6 mRNA expression levels of AC genotype chicken were significantly higher than the other groups (P＜0.05).The TNF-α mRNA expression levels of AC genotype chicken were significantly higher than the other groups when cCHMPS was 100 μg/mL (P＜0.05).There results indicated that the cCHMPS could stimulate NF-κB,TNF-α,IL 6 mRNA expression in different MHC B-Lβ Ⅱ genotype chickens,and the optimum immunomodulatory does were different in each MHC B-Lβ Ⅱ genotype chicken.关键词
中药复方多糖/MHC B-LβⅡ基因型/鸡淋巴细胞/细胞因子Key words
compound Chinese herbal medicines polysaccharides (cCHMPS)/MHC B-Lβ Ⅱ genotype/chicken lymphocyte/cytokines分类
生物科学引用本文复制引用
马昭,朱晓庆,杨莉,刘晓婷,张保军,谷新利,商云霞..中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞中NF-κB、TNF-α、IL-6mRNA表达的影响[J].中国畜牧兽医,2017,44(12):3446-3452,7.基金项目
国家自然科学基金(31460673) （31460673）
