中国畜牧兽医2017，Vol.44Issue(12)：3466-3472,7.DOI:10.16431/j.cnki.1671-7236.2017.12.011
猪嵴病毒病原特性及检测技术研究进展
Research Advances in Pathogenicity and Detection Techniques of Porcine Kobuvirus
摘要
Abstract
Porcine kobuvirus (PKV) is a member of the newly detected in the genus Kobuvirus,family Picornaviridae in healthy pigs and diarrhea pigs.It may cause diarrhea of pigs and cause significant economic losses to pig industry.At present,the studies find that,PKV is widely distributed in pigs and has been detected in diarrhea and clinically healthy pigs,and the positive rate of the virus varies from 3.9％ to 100.0％.A typical porcine kobuvirus virion is 30 nm in diameter,and its genome is 8 120 bp in length and contains a single open reading frame (ORF) encoding a protein of 2 488 amino acids.The genome organization is typical for picornaviruses:A 5'UTR,a leader (L) protein,structural proteins P1 (VP0,VP3 and VP1),non-structural proteinsP2 (2A,2Band 2C) and P3 (3A,3B,3C and 3D),a 3' UTR and a Poly (A) tail.There are 30 amino acid deletions in the 2B coding region of the porcine kobuvirus,VP1 protein is the most frequent variant of the small RNA virus,structural protein,which contains the main epitope,can promote the body to produce neutralizing antibodies.At present,the methods of detecting PKV are reverse transcription polymerase chain reaction (RT-PCR),TaqMan probe Realtime RT-PCR and reverse transcription-loop mediated amplification (RT-LAMP).In this paper,a brief overview of the taxonomy,epidemiological profile,genome structure,genetic characteristics and detection techniques of PKV was made,so as to provide references for the further study and understand PKV.关键词
猪嵴病毒(PKV)/流行病学/基因组结构/遗传特性/检测技术Key words
porcine kobuvirus (PKV)/epidemiology/genome structure/genetic characteristics/detection technique分类
农业科技引用本文复制引用
黄晓星,王仙,金文杰..猪嵴病毒病原特性及检测技术研究进展[J].中国畜牧兽医,2017,44(12):3466-3472,7.基金项目
江苏省“青蓝工程” （）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江苏省农业三新工程(SXGC[2015]077) （SXGC[2015]077）
常州市科技支撑项目(CE20152006) （CE20152006）
