中国畜牧兽医2017，Vol.44Issue(12)：3497-3504,8.DOI:10.16431/j.cnki.1671-7236.2017.12.015
利用体外产气法评定不同比例氨化秸秆替代苜蓿的组合效应
Evaluation of Associative Effects of Alfalfa Meal and Ammoniated Corn Straw by Gas Production Technique in vitro
摘要
Abstract
This experiment was conducted to determine the best roughage combination of alfalfa meal (AM) and ammoniated corn straw (ACS),so as to improve the efficiency of roughage utilization and reduce the cost of feeding.The AM was mixed with ACS in the proportion of 100 ∶ 0,80 ∶ 20,60 ∶ 40,50 ∶ 50,40 ∶ 60,20 ∶ 80 and0 ∶ 100 with3 replicates per group.The accumulated gas production (GP) at fermentation for 3,6,12,24,48 h,and the changes of p H,dry matter disappearance rate (DMD),ammonia nitrogen (NH3-N) concentration,microbial protein (MCP) and volatile fat acids (VFA) concentration,and their single factor associative effects index (SFAEI) and multiply factors associative effects index (MFAEI) were determined by gas production technique in vitro after fermentation for 48 h.The results showed as follows:The accumulated gas production of AM20 ∶ ACS80 group was higher than that of the other groups,and the DMD of AM0 ∶ ACS100 group was significantly lower than other groups (P＜0.05).The NH3-N concentration of AM20 ∶ ACS80 and AM0 ∶ ACS100 groups was significantly higher than the other groups (P＜0.05).The MCP concentration was highest in AM20 ∶ ACS80 group,and the concentration of acetic acid and TVFA in AM20 ∶ ACS80 group was the highest (P＜0.05),and the acetic acid/propionic acid ratio in all groups was more than 3,which indicating that it belonged to the acetic acid fermentation type.The pH during the fermentation ranged from 6.69 to 6.85.The results of MFAEI and SFAEI of each combination indicated that only the AM20 ∶ ACS80 group showed the positive associative effects.In conclusion,the 20 ∶ 80 combination ratio for AM and ACS showed the best associative effects.关键词
苜蓿/氨化秸秆/饲料组合效应/瘤胃发酵参数Key words
alfalfa meal (AM)/ammoniated corn straw (ACS)/associative effects/rumen fermentation parameters分类
农业科技引用本文复制引用
于满满,姜雨轩,张美美,谢小来,王晨曦..利用体外产气法评定不同比例氨化秸秆替代苜蓿的组合效应[J].中国畜牧兽医,2017,44(12):3497-3504,8.基金项目
农业部公益性行业(农业)科研专项经费项目项目(201503134) （农业）
