中国畜牧兽医2017，Vol.44Issue(12)：3543-3553,11.DOI:10.16431/j.cnki.1671-7236.2017.12.022
哈萨克羊DQB2基因外显子2多态性及遗传变异分析
Polymorphisms and Its Genetic Variation Analysis of DQB2 Gene Exon 2 in Kazakh Sheep
摘要
Abstract
This study was aimed to investigate the association between the polymorphism of DQB2 gene exon 2 and the susceptibility to Kazakh sheep brucellosis.The DQB2 gene exon 2 of Kazakh sheep lymphocyte antigen was amplified by PCR-SSCP method from 146 healthy and 28 infected with Brucella Kazakh sheep,and the single nucleotide polymorphisms (SNP) was analyzed,then the different alleles were selected for cloning and sequencing.In order to analyze its correlation with brucellosis susceptibility,the differences in gene frequency and genotype frequency of each SNP locus were analyzed by Chi-square test.Bioinformatics softwares were used to analyze the secondary structure of mRNA,the secondary structure,tertiary structure and epitope of protein.The sequencing result showed that 33 SNPs were detected in 270 bp DNA sequence,the gene frequencies of C9G and A180G were extremely significantly different in case group and control group (P＜0.01),and its genotype frequencies presented significantly difference (P＜0.05).Similarly,A13T and C133G loci were significant difference in case group and control group (P＜0.05).Further analysis result showed that the minimum free energy of the A180G mutation site was the lowest and its mRNA secondary structure was the most stable;Both A13T and C133G mutation sites caused the changes of mRNA secondary structure,protein secondary,tertiary structure and antigenic epitope of protein,respectively.The results showed that the polymorphism of DQB2 gene exon 2 might be significantly correlated with brucellosis susceptibility in Kazakh sheep.关键词
DQB2基因/PCR-SSCP/SNPs/布鲁氏菌病/易感性/生物信息学Key words
DQB2 gene/PCR-SSCP/SNPs/brucellosis/susceptibility/bioinformatics分类
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王元元,严国,罗成,齐江姣,周光普,谭君,高剑峰..哈萨克羊DQB2基因外显子2多态性及遗传变异分析[J].中国畜牧兽医,2017,44(12):3543-3553,11.基金项目
国家重点基础研究发展计划(973计划)(2010CB530200) （973计划）
