中国畜牧兽医2017，Vol.44Issue(12)：3563-3569,7.DOI:10.16431/j.cnki.1671-7236.2017.12.024
抑制UCHL1对猪卵母细胞体外成熟、透明带泛素化及多精入卵的影响
Effects of UCHL1 Inhibition on Porcine Oocyte Matuation in vitro, Zona Pellucida Ubiquitination and Polyspermy
摘要
Abstract
This study was aimed to examine the effects of UCHL1 inhibition on porcine oocyte maturation in vitro,zona pellucida (ZP) ubiquitination and polyspermy.DAPI staining,Hoechst staining and SDS-PAGE methods were used to detect the matuation rate of porcine oocytes in vitro,the level of ubiquitination of zona pellucida (ZP) and polyspermy.The results showed that after different concentrations UCHL1 inhibitor (10,20,25 and 30 μmol/L,DMSO and control group) were added into maturation medium for culturing 46 h in vitro,the mature rate of control group was 86.22％,while the maturation rate of 30μmol/L group was 15.30％,and the maturation rate of every treatment group had significant difference (P＜0.05).Western blotting result showed that every group generated ubiquitin markers of ZP were about 61,80 and 106 ku in different degree.According to the analysis of gray value,the result had significant difference (P＜0.05).Conducting fertilization in vitro,the number of sperm adhered on oocyte ZP in control group was the most,the number of sperm running into oocyte was fewer,the number of sperm adhered on oocyte ZP with 30 μmol/L UCHL1 inhibitor was the fewest,and there was almost no sperm running into the oocyte.The results showed that UCHL1 inhibitor had an impact on maturation of porcine oocytes in vitro.With the higher concentration of UCHL1,the lower degree of ZP protein ubiquitinated,UCHL1 could regulate sperm attachment and polyspermy.关键词
猪卵母细胞/UCHL1抑制剂/体外成熟/透明带泛素化/多精入卵Key words
porcine oocyte/UCHL1 inhibitor/IVM/ZP ubiquitination/polyspermy分类
农业科技引用本文复制引用
史文姝,金一..抑制UCHL1对猪卵母细胞体外成熟、透明带泛素化及多精入卵的影响[J].中国畜牧兽医,2017,44(12):3563-3569,7.基金项目
国家自然科学基金(31260529) （31260529）
吉林省科技厅重点科技攻关项目(20150204079NY) （20150204079NY）
