中国畜牧兽医2017，Vol.44Issue(12)：3570-3577,8.DOI:10.16431/j.cnki.1671-7236.2017.12.025
驯鹿干扰素β1基因的克隆及遗传进化分析
Cloning and Phylogenetic Analysis of IFNβ1 Gene of Reindeer
摘要
Abstract
This study was aimed to clone the full-length sequence of interferon beta 1 (IFNβ1) gene from the top of reindeer antler,and analyze its molecular characteristics,to lay a foundation for its biological activity and practical application.One pair of primers was designed based on the conserved sequence of IFNβ1 gene of cattle and sheep published in GenBank.IFNβ1 gene of reindeer was amplified by PCR from genome DNA extracted from mesenchyme of reindeer antler tip,and the product was cloned and sequenced.The result showed that the full length sequence of reindeer IFNβ1 gene was 561 bp,encoded 186 amino acids and contained 3 glycosylation sites.The encoded protein contained4 alpha helixes,4 beta fold zones and 7 beta turn angles.Compared the amino acids of IFNβ1 gene with other mammal species,the homology was 45.1％ to 92.0％.The results of sequence andphylogenetic tree analysis showed that IFNβ1 gene had species diversity,the relationship was proportional to homology.This result laid the foundation for further research on the expression,biological activity and application of reindeer IFNβ1 gene.关键词
驯鹿/IFNβ1基因/序列分析/遗传进化Key words
reindeer/IFNβ1 gene/sequence analysis/genetic evolution分类
生物科学引用本文复制引用
翟健程,王强辉,夏彦玲,李和平..驯鹿干扰素β1基因的克隆及遗传进化分析[J].中国畜牧兽医,2017,44(12):3570-3577,8.基金项目
国家林业局珍稀濒危物种野外救护与繁育项目(2012) （2012）
中央高校基本科研业务费专项资金项目(2572016AA42) （2572016AA42）
