中成药2017，Vol.39Issue(12)：2469-2474,6.DOI:10.3969/j.issn.1001-1528.2017.12.005
尿感方清除受感染膀胱上皮细胞胞内尿道致病性大肠杆菌的作用
Effect of Niaogan Decoction on expulsion of uropathogenic Escheriehia coli from human bladder epithelial cells
摘要
Abstract
AIM To observe the effects of Niaogan Decoction (Portulacae Herba,Taraxaci Herba) on expulsion of uropathogenic Escheriehia coli (UPEC) from human bladder epithelial cells and to explore its mechanism.METHODS After HTB-9 was infected with UPEC,drug urine exocytosed the UPEC invaded into urinary bladder epithelial cell were observed.With molecular biology technology,the mechanism of key factors to TLR4/cAMP signal pathway such as Toll-like receptor 4 (TLR4),adeuylate cyclase 3 (AC3),cyclic adenosine monophosphate (cAMP),protien kinase A (PKA),Myosin Ⅶ A and Rab interacting protein (MyRIP),Rab27b and Caveolin-1 were analyzed.RESULTS Treatment with Niaogan Decoction resulted in a significant increase in UPEC exocytosis compared with the blank control high dose group.Niaogan Decoction group improved TLR4,AC3,MyRIP,Rab27b and Caveolin-1 protein expression,increased intracellular cAMP content and promoted PKA activation.CONCLUSION Niaogan Decoction has the capacity to exocytose UPEC,its mechanism may relate to TLR 4/cAMP signal pathway.关键词
尿感方/尿道致病性大肠杆菌/胞吐/人膀胱上皮细胞/TLR4/cAMP信号传导通路Key words
Niaogan Decoction/UPEC/expulse/human urinary bladder epithelial cell/TLR4/cAMP signal pathway分类
医药卫生引用本文复制引用
吴雨,蒋健,贺敏,张磊阳,陈文文..尿感方清除受感染膀胱上皮细胞胞内尿道致病性大肠杆菌的作用[J].中成药,2017,39(12):2469-2474,6.基金项目
国家自然科学基金资助项目(81403168) （81403168）
