中成药2017，Vol.39Issue(12)：2475-2480,6.DOI:10.3969/j.issn.1001-1528.2017.12.006
大黄蛰虫丸对小鼠酒精性肝纤维化损伤的保护作用
Protective effects of Dahuang Zhechong Pills on mice with alcohol-induced liver fibrosis
摘要
Abstract
AIM To investigate the protective effects of Dahuang Zhechong (DHZC) Pills (Rhei Radix et Rhizoma,Eupolyphaga seu Steleophaga,Hirudo,etc.) against alcoholic liver fibrosis (ALF) injury in mice and to explore the underlying mechanisms.METHODS C57BL/6 male mice were used to build up ALF injury model,intervened with DHZC Pills.The serum of mice was examined for changes in alanine transaminase (ALT),aspartate aminotransferase (AST),interleukin-6 (IL-6),interleukin-10 (IL-10),interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α).Simultaneously,the deposit of collagen 1 (COL-1) and apoptotic cell death in liver tissues were analyzed by immunofluorescent and TUNEL assay,respectively.The expressions of cleaved caspase-3 (CC3) in livers were measured by Western blot.RESULTS Compared with the model group,the levels of serum ALT,AST,IL-6,IFN-γand TNF-α of mice in DHZC group were decreased significantly.And the level of serum IL-10 of mice in DHZC group was increased significantly.Mice in DHZC group had higher rates of COL-1 deposition and apoptotic cell death in liver tissues than those in the model group.Mice treated with DHZC Pills showed lower expression of CC3.CONCLUSION DHZC Pills confers protection against ALF injury in mice by inhibiting the generation of COL-1 and down-regulating apoptosis of liver cells death as a result of adjusting the levels of inflammatory factors.关键词
大黄蛰虫丸/酒精性肝纤维化损伤/保护作用Key words
Dahuang Zhechong Pills/alcoholic liver fibrosis injury/protective effect分类
医药卫生引用本文复制引用
钟伟超,周楚莹,高磊,吕志平,黄少慧..大黄蛰虫丸对小鼠酒精性肝纤维化损伤的保护作用[J].中成药,2017,39(12):2475-2480,6.基金项目
国家青年科学基金(81302948,81603501) （81302948,81603501）
