中成药2017，Vol.39Issue(12)：2521-2524,4.DOI:10.3969/j.issn.1001-1528.2017.12.015
HPLC法同时测定灯盏细辛注射液中10种成分
Simultaneous determination of ten constituents in Dengzhan Xixin Injection by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of 5-caffeoylquinic acid,chlorogenic acid,4-caffeoylquinic acid,caffeic acid,1,3-dicaffeoylquinic acid,scutellarin,isochlorogenic acid B,3,5-dicaffeoylquinic acid,apigenin-7-O-glucronide and 4,5-dicaffeoylquinic acid in Dengzhan Xixin Injection [Erigeron breviscapus (Vaniot.) Hand.-Mazz].METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Inertsil(R) ODS-3 column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.4％ phosphoric acid-acetonitrile flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelength was set at 327 nm.RESULTS Ten constituents showed good linear relationships within their own ranges (r≥0.999 0),whose average recoveries were 94.11％-100.64％ with the RSDs of 1.12％-2.77％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dengzhan Xixin Injection.关键词
灯盏细辛注射液/化学成分/HPLCKey words
Dengzhan Xixin Injection/chemical constituents/HPLC分类
医药卫生引用本文复制引用
刘光丽,徐文龙,王张..HPLC法同时测定灯盏细辛注射液中10种成分[J].中成药,2017,39(12):2521-2524,4.基金项目
四川省应用基础研究计划重点项目(2016JY0017) （2016JY0017）
