广东海洋大学学报2017，Vol.37Issue(6)：7-10,4.DOI:10.3969/j.issn.1673-9159.2017.06.002
基于18S-ITS1-5.8S序列的吉富罗非鱼群体遗传多样性分析
Genetic Diversity of GIFT Strains of Oreochromis niloticus Based on 18S-ITS1-5.8S Sequence
摘要
Abstract
18S-ITS1-5.8S sequences of the GIFT strains of Oreochromis niloticus were amplified by PCR. The result was used to analyze the genetic variation of the 20th and 21st generation. The study showed that the sequences of 18S, ITS1, 5.8S were 156, 483 - 540, and 64 bp, respectively, and the main variable sites were located in ITS1. Based on ITS1 sequences, the 20th generation (17 individuals) genetic distance was 0.001. It contained 530 conservative sites, 10 variable sites, 4 haploid types. The haplotype diversity was 0.331±0.143, and nucleotide diversity was 0.002, and the average nucleotide difference was 1.279. The 21th generation (42 individuals) genetic distance was 0.015, and some nucleotides have severely been lost in 6 individuals. It contained 492 conservative sites, 49 variable sites and 12 haploid types. It haplotype diversity was 0.638±0.083, nucleotide diversity was 0.014, and the average nucleotide difference was 6.945. The results indicated that the variation of ITS1 sequences were relatively small and genetic diversity was at a low level in the two generations of GIFT strains of Oreochromis niloticus.关键词
吉富罗非鱼/遗传多样性/18S-ITS1-5.8S序列Key words
GIFT/Genetic diversity/18S-ITS1-5.8S sequence分类
生物科学引用本文复制引用
袁吉贵,李爵乾,刘丽,陈增祥,张艳苹..基于18S-ITS1-5.8S序列的吉富罗非鱼群体遗传多样性分析[J].广东海洋大学学报,2017,37(6):7-10,4.基金项目
中央分成海域使用金支出项目(2011-2-2-09-3),广东省海洋渔业科技推广专项(A201308E02),茂名市特色农产品广东省农业科技园区(2014B020222003-8) （2011-2-2-09-3）
