广东海洋大学学报2017，Vol.37Issue(6)：26-32,7.DOI:10.3969/j.issn.1673-9159.2017.06.005
低盐胁迫对钝吻黄盖鲽幼鱼鳃Na+/K+-ATP酶、肝脏抗氧化酶和非特异免疫酶的影响
Influence of Low Salinity Stress on the Gill Na+/K+-ATPase, Liver Antioxidase and Non-Specific Immune Enzyme in Juvenile Pleuronectes yokohama
摘要
Abstract
The adaptations to low salinity stress, the mechanism of antioxidation and the ability of non-specific immunity in Pleuronectes yokohama were investigated by measuring the superoxide dismutase (SOD), catalase (CAT), acid phosphatase (ACP) and alkaline phosphatase (AKP) activities in the liver, and Na+/K+-ATPase (NKA) in the gill. The two low salinity groups, salinity 6 and 18, were denoted as S6 and S18. The results showed that NKA activities of experimental groups increased initially and then decreased over time. The activities reached the maximum level on 1 d (P < 0.05) in both the S6 and S18 groups. NKA activity of S6 group dropped after 7 d, while that of the S18 group began to decline sharply from 3 d to 14 d. The SOD and CAT activities in the liver both increased initially and then decreased over time. The SOD activities in the S6 and S18 groups both increased to the maximum on 7d (P < 0.05), but the S6 group remained at a higher level on 14 d, whereas the S18 group had lower level of enzyme activity. As for the CAT activities, the S6 group and S18 group all reached the maximum on 7 d, and restored to 0 d level on 14 d. The liver ACP activities in the S6 and S18 groups also increased to the highest on 3 d. On 14 d, the S18 group dropped to the level of the control group, while the AKP activity in the S18 group increased initially and then declined. The AKP activity of the S6 and S18 groups was at the maximum on 3 d. The results of this study revealed that low salinity stress had remarkable effects on the NKA activity, antioxidant and non-specific immunity mechanism of the juvenile P. yokohama. The result also indicated that the fish showed strong adaptability when they were cultured at salinity 18.关键词
钝吻黄盖鲽/盐度/Na+/K+-ATP酶/抗氧化酶/非特异免疫Key words
Pleuronectes yokohama/salinity/Na+/K+-ATPase/antioxidase/non-specific immunity分类
农业科技引用本文复制引用
崔前进,陈冰,邱丽华,付旭,杨博学,韩雨哲,姜晨..低盐胁迫对钝吻黄盖鲽幼鱼鳃Na+/K+-ATP酶、肝脏抗氧化酶和非特异免疫酶的影响[J].广东海洋大学学报,2017,37(6):26-32,7.基金项目
广东省生态环境重点实验室开放基金(LFE-2016-4) （LFE-2016-4）
