广东海洋大学学报2017，Vol.37Issue(6)：89-95,7.DOI:10.3969/j.issn.1673-9159.2017.06.014
高温胁迫对2种杂交石斑鱼存活率及血清生化指标的影响
Influence of High Temperature Stress on Survival Rate and Serum Biochemical Indexes of 2 Epinephelus Hybrids
摘要
Abstract
Hybrid of Epinephelus moara♀× E.lanceolatus♂ (i.e.Yunlong:BL33.5 ± 2.4 cm and BW 584.15 ± 10.35 g), and hybrid of E. coioides♀× E. lanceolatus♂ (i.e. Qinglong: BL 25.1 ± 2.2 cm and BW 375.41 ± 7.35 g) were selected to investigate their tolerance to high temperature cultured condition. The fishes were cultured at the initial water temperature of 25 ℃ with a daily increment of 1 ℃. During the high temperature culture period, the survival rates were monitored and the median lethal temperature (LT50) for the hybrids was estimated. In addition, the changes in several serum biochemical parameters were determined at 25, 29, 33 and 36 ℃. The results indicated that the LT50 of Yunlong and Qinglong were 34.9 and 37.9 ℃, respectively. For the Qinglong hybrid, serum concentration of triglycerides (TG) and glucose (GLU), the activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) showed a significant increase with increasing cultured temperatures (P<0.01). In contrast, serum concentration of creatinine (CREA) and total cholesterol (T-CHO) decreased initially and then increased (P < 0.01). Other serum biochemical indices such as glutamic-oxaloacetic transaminase (GOT), glutamate pyruvate transaminase (GPT) and catalase (CAT) showed no significant changes (P>0.05). For Yunlong hybrid, serum concentration of T-CHO and GLU elevated significantly (P < 0.01) with the increase of culture water temperature, while the serum CREA and TG levels increased initially and then decreased at the end (P <0.05). The serum activities of GOT, ACP and AKP showed a descending trend (P < 0.05) whereas the changes in serum activities GPT and CAT were insignificant (P<0.05).关键词
杂交石斑鱼/云纹石斑鱼/斜带石斑鱼/鞍带石斑鱼/子一代/高温胁迫/存活率/血清生化指标Key words
hybrid grouper/Epinephelus moara♀/Epinephelus coioides/Epinephelus lanceolatus/F1/high temperature stress/survival rate/biochemical indexes分类
农业科技引用本文复制引用
邵彦翔,陈超,张廷廷,李炎璐,张梦淇,李文升..高温胁迫对2种杂交石斑鱼存活率及血清生化指标的影响[J].广东海洋大学学报,2017,37(6):89-95,7.基金项目
中国-东盟海上合作基金项目(2016-2018)山东省自然科学基金项目(ZR2015PC015)、青岛市市南区科技项目(2014-14-011-SW) （2016-2018）
