浙江医学2017，Vol.39Issue(21)：1865-1867,1871,4.DOI:10.12056/j.issn.1006-2785.2017.39.21.2017-1602
抗细胞膜DNA抗体在系统性红斑狼疮诊断中的应用价值
Diagnostic value of anti-cell membrane DNA antibodies for systemic lupus erythematosus
摘要
Abstract
Objective To assess the diagnostic value of anti-cell membrane DNA (mDNA) antibodies for systemic lupus erythematosus (SLE).Methods The serum samples from 137 SLE patients (SLE group),72 non-SLE autoimmune diseases patients(non-SLE group) and 84 healthy subjects (control group) were collected.The antinuclear antibody (ANA) and anti-mDNA antibody were detected by indirect immunofluorescence assay,anti-double-stranded DNA (dsDNA) was detected by ELISA,ribosomal P protein (Rib-P) antibody,anti-Smith (Sm) antibody and anti-nucleosome antibody (AnuA) were detected by immunoblotting (IBT).The diagnostic values of above indicators were assessed.Results The positive rate of anti-mDNA antibody in SLE patients(62.8％) was significantly higher than that in non-SLE groups(12.5％) and control group(2.4％),which was significantly higher than that of anti-ds-DNA antibodies (40.9％),Rib-P antibody (21.2％),anti-Sm antibody (18.2％) and AnuA (17.5％)(all P＜0.05).The sensitivity of combination of anti-mDNA antibody with anti-ds-DNA antibody,Rib-P antibody,anti-Sm antibody or AnuA for diagnosis of SLE was all significantly higher than that of anti-mDNA alone (all P＜0.01).The positive rate of anti-mDNA antibody was 65.9％ in SLE patients with other antibodies negative.The combination of anti-mDNA antibody with other antibodies significantly improved the detection rate of SLE (87.6％).Conclusion Anti-mDNA antibody is a sensitive serological marker of SLE,combination of anti-mDNA antibody with other indicators may further improve the sensitivity in diagnosis of SLE.关键词
抗细胞膜DNA抗体/系统性红斑狼疮/灵敏度/特异度Key words
Anti-cell membrane DNA antibody/Systemic lupus erythematosus/Sensitivity/Specificity引用本文复制引用
戚建锋,吴翡斐,施金俏,翁巍,王文琴,王利民..抗细胞膜DNA抗体在系统性红斑狼疮诊断中的应用价值[J].浙江医学,2017,39(21):1865-1867,1871,4.基金项目
金华市科技计划项目(2014-3-074) （2014-3-074）
