浙江医学2017，Vol.39Issue(21)：1872-1876,5.DOI:10.12056/j.issn.1006-2785.2017.39.21.2017-548
盆腔神经损伤与IL-10相互作用在子宫腺肌病中的研究
Injury of pelvic nerves and serum level of IL-10 in patients with adenomyosis
摘要
Abstract
Objective To investigate the damage of pelvic nerves and the serum levels of IL-10 in patients with adenomyosis.Methods Histological sections of the uterosacral ligaments and the isthmus of fallopian tubes were obtained from women undergoing hysterectomy for adenomyosis (adenomyosis group,n=33) and leiomyoma or cervical intraepithelial neoplasia Ⅲ (CIN Ⅲ) (non-adenomyosis group,n=32).The expression of protein gene product 9.5(PGP 9.5) in uterosacral ligaments and the isthmus of fallopian tube was immunohistochemically analyzed.The distribution of nerve fibers in the uterosacral ligaments and the isthmus of fallopian tube was examined microscopically.Serum IL-10 levels were determined by enzyme linked immunosorbent assay (ELISA) in two groups.Results Varying degrees of injuries and interruptions of the uterosacral ligaments were noted in the adenomyosis patients and there was strong evidence of neovascularization of the peritoneal surfaces over the ligament,adjacent peritoneum and mesosalpinx.The expression of PGP 9.5 and the density of nerve fibers in the mucosal and muscular layers of fallopian tubes were significantly decreased in adenomyosis (all P＜0.05).The nerve fibers stained with PGP 9.5 in the serous layer of the fallopian tube among the two groups showed no significant difference(both P ＞0.05).The nerve fibers were decreased in the uterosacral ligaments of adenomyosis group compared with non-adenomyosis group (P＜0.05).The abnormal hyperplasia of the nerves was observed and the surrounded injured arterioles had thickened vessel walls and narrowed vascular cavity in adenomyosis group.The level of serum IL-10 in patients with adenomyosis was significantly higher than that in non-adenomyosis patients (P＜0.05).Conclusion Varying degrees of injuries to the nerves in the uterosacral ligaments and in the mucosal and muscular layers of fallopian tubes are noted in the adenomyosis patients.There is significantly abnormal serum IL-10 in patients with adenomyosis,which indicates that the interaction between pelvic nerve injury and inflammation may be responsible for the disease development.The results may provide a novel insight into the pathogenesis of adenomyosis.关键词
子宫腺肌病/宫骶韧带/神经纤维/神经损伤/IL-10Key words
Adenomyosis/Uterosacral ligament/Nerve fibers/Injured nerve/IL-10引用本文复制引用
蔡瑶瑶,徐欣欣,吴雪清..盆腔神经损伤与IL-10相互作用在子宫腺肌病中的研究[J].浙江医学,2017,39(21):1872-1876,5.基金项目
温州市公益性科技计划项目(Y20140743) （Y20140743）
