浙江医学2017，Vol.39Issue(21)：1883-1886,后插1,5.DOI:10.12056/j.issn.1006-2785.2017.39.21.2017-1672
麻杏石甘汤对哮喘模型大鼠肺组织STAT4及其mRNA表达的影响
Expression of STAT4 in asthma rats treated with Chinese medicine Maxingshigan Decoction
虞琳 1潘建伟 1叶乐平2
作者信息
- 1. 322000义乌,浙江大学医学院附属第四医院儿科
- 2. 温州医科大学附属第二医院儿童呼吸科
- 折叠
摘要
Abstract
Objective To investigate the expression of STAT4 in asthma rats treated with Chinese medicine Maxingshigan Decoction.Methods Fifty five SPF SD rats were randomly divided into 5 groups:control group (group A),asthma group(group B),high-dose,medium-dose and low-dose Maxingshigan Decoction treatment groups (group C,group D,group E).The rat model of asthma was established by ovalbumin (OVA) challenge methods.The bronchoalveolar lavage fluid (BALF) and lung tissues were obtained.The concentrations of IL-12 and IL-13 in BALF were measured by ELISA,the inflammation of airways was observed by HE staining;the expressions of STAT4 protein and mRNA were detected by immunohistochemistry and in situ hybridization,respectively.Results Compared with the control group,the concentration of IL-13 in BALF of asthma group was higher,while it was decreased in Maxingshigan Decoction treatment groups of asthma rats.On the contrary,the concentration of IL-12 in BALF was lower in asthma group than that in control group.The IL-12 levels in BALF were increased in Maxingshigan Decoction groups,and compared with asthma rats,the levels in groups C and D were significantly elevated(P＜0.05).HE staining showed airway smooth muscle and vascular muscle hyperplasia and peripheral inflammatory cells infiltration in asthma rats.Maxingshigan Decoction dose-dependently alleviated the pathological changes in asthma rats.STAT4 and its mRNA were expressed in airway smooth muscles and vascular muscle layers in all groups.The expression of STAT4 in Maxingshigan Decoction groups was higher than that in control group,and the expression of STAT4 protein in group C was significantly higher than that in asthma group (P＜0.05).The expression of STAT4 mRNA in groups C and D were significantly up-regulated compared with the asthma group (P＜0.05).Conclusion Chinese medicine Maxingshigan Decoction can reduce airway inflammation in asthma rats through up-regulation of STAT4 expression.关键词
哮喘/麻杏石甘汤/STAT4/STAT4/mRNAKey words
Asthma/Maxingshigan Decoction/STAT4/STAT4 mRNA引用本文复制引用
虞琳,潘建伟,叶乐平..麻杏石甘汤对哮喘模型大鼠肺组织STAT4及其mRNA表达的影响[J].浙江医学,2017,39(21):1883-1886,后插1,5.
