浙江医学2017，Vol.39Issue(22)：1948-1952,5.DOI:10.12056/j.issn.1006-2785.2017.39.22.2016-228
单纯疱疹病毒Ⅱ型感染对生殖道上皮细胞间紧密连接和单核细胞趋化性影响的初步研究
Effect of HSV-2 infection on tight junction of genital epithelial cells and chemotaxis of monocytes
摘要
Abstract
Objective To investigate the effect of human herpes simplex virus type Ⅱ (HSV-2) on intercellular tight junction of genital epithelial cells and the chemotaxis of monocytes.Methods The in vitro genital epithelial tight junction monolayer model based on HEC-1-A Millicell culture was developed.After infected with low dose HSV-2 (MOI=0.1),the cell viability was tested and the transepithelial electrical resistance was measured at different time points to evaluate intercellular tight junction of HEC-1-A cells.The expression of ZO-1 protein was detected with Western blot and RT-PCR.The in vitro monocyte chemotaxis model was used through inducing U937 cell to express oligopeptide chemotaxis receptor,and monocyte chemotaxis was detected after HSV-2 infected HEC-1-A culture supernatant was added.Results The transepithelial electrical resistance of HEC-1-A cells was reduced by HSV-2 infection (MOI=0.1) gradually,especially in the middle and late period (P＜0.01),and the cell viability had no significant changes (above 80％).HSV-2 infection down-regulated the protein expression of ZO-1 in HEC-1-A cells,but had no effect on E-cadherin and Occuldin expression (P ＞0.05).The supernatant from HSV-2 infected HEC-1-A cells culture strongly induced monocyte chemotaxis (P＜0.01).Conclusion Low dose HSV-2 infection can reduce transepithelial electrical resistance of HEC-1-A cells and damage cell tight junction,which may be related to down-regulation of ZO-1 protein;and also induce the chemotaxis of monocytes by release of chemotactic factors.关键词
单纯疱疹病毒Ⅱ型/紧密连接/趋化反应Key words
Herpes simplex virus type Ⅱ (HSV-2)/Tight junction/Chemotaxis引用本文复制引用
吕晓文,邓琳,楚鹰,黄先玫..单纯疱疹病毒Ⅱ型感染对生殖道上皮细胞间紧密连接和单核细胞趋化性影响的初步研究[J].浙江医学,2017,39(22):1948-1952,5.基金项目
南京医科大学科技发展基金面上项目(2015NJMU175) （2015NJMU175）
