浙江医学2017，Vol.39Issue(22)：1953-1956,1975,5.DOI:10.12056/j.issn.1006-2785.2017.39.22.2017-990
高脂诱导胰岛素抵抗大鼠对不同脂肪组织水通道蛋白7的影响
Effects of aquaporin 7 in different adipose tissues of rats with insulin resistance
摘要
Abstract
Objective To investigate the expression of aquaporin 7(AQP7) in different adipose tissues of insulin resistance rats.Methods Male SD rats were randomly divided into insulin resistant and control groups with 10 animals in each.The insulin resistance model was induced by feeding with high-fat diet.The changes of body weight,serum triglyceride,cholesterol,high density lipoprotein,low density lipoprotein,glycerol,fasting blood glucose,insulin and adipokines in two groups were observed.The expression of AQP7 mRNA and protein,and the expression of PKB and phosphorylated protein in subcutaneous adipose tissue (SAT) and epididymal adipose tissue (visceral adipose tissue,VAT) were detected and compared.Results Compared to control group,the serum low density lipoprotein,fasting blood glucose,insulin,IL-6,TNF-α and HOMA-IR in insulin resistance group were higher(P＜0.01 or P＜0.05),while high density lipoprotein and adiponectin were lower(P＜0.05) at 12th week after high diet feeding.The level of AQP7 expression in VAT was higher than that in SAT,and the expression of AQP7 in VAT of insulin resistance group was higher than that of control group in both gene and protein level.The expression of PKB phosphorylated protein in VAT was lower than that in SAT,and the expression level in insulin resistance group was lower than that in control group.Conclusion The abnormal expression of AQP7 in visceral adipose tissue may be related to the occurrence of insulin resistance.关键词
水通道蛋白7/脂肪组织/胰岛素抵抗Key words
AQP7/Adipose tissue/Inulin resistance引用本文复制引用
潘伟,沈飞霞,谷雪梅..高脂诱导胰岛素抵抗大鼠对不同脂肪组织水通道蛋白7的影响[J].浙江医学,2017,39(22):1953-1956,1975,5.基金项目
国家自然科学基金青年资助项目(81000356) （81000356）
浙江省自然科学基金(Y2080418) （Y2080418）
