浙江医学2017，Vol.39Issue(22)：1957-1960,1980,5.DOI:10.12056/j.issn.1006-2785.2017.39.22.2016-2092
沙利度胺抑制实验性自身免疫性脑脊髓炎与促进Treg细胞中枢募集有关
Thalidomide alleviates experimental autoimmune encephalomyelitis through promoting Treg cell recruitment in rats
摘要
Abstract
Objective To investigate the effect of thalidomide on experimental autoimmune encephalomyelitis(EAE)in rats and its related mechanisms.Methods SD rates were injected with spinal cord homogenate of guinea pig (EAE group) or solvent (control group),then the rats were treated with thalidomide at different doses (15mg/kg,30mg/kg,60mg/kg).The clinic scores of EAE were assessed daily until animals were sacrificed.All rats were sacrificed at d20 after model induced and paraffin sections were prepared and stained with hematoxylin-eosin.Serum INF-γ and TNF-α levels were measured by ELISA.The spinal cord infiltrating CD4+T cells and CD4+CD25+T cells were determined by flow cytometry.Results Thalidomide 15mg/kg did not effectively reduce the severity of EAE,but reduced the TNF-α protein levels.Thalidomide 30mg/kg and 60mg/kg reduced the clinic score of EAE,the inflammation in the spinal cord,the number of infiltrating CD4+T cells and the INF-γ,TNF-α protein levels.And thalidomide 60mg/kg promoted CD4+CD25+T cell recruitment into central nervous system.Conclusion Thalidomide alleviated the severity of EAE in rats,which may be related to the promotion of CD4+CD25+T cell recruitment into central nervous system.关键词
沙利度胺/多发性硬化/实验性自身免疫性脑脊髓炎/调节性T细胞Key words
Thalidomide/Multiple sclerosis/EAE/Treg cells引用本文复制引用
王赵伟,厉芳,吴承龙,肖桂荣,钟芳芳..沙利度胺抑制实验性自身免疫性脑脊髓炎与促进Treg细胞中枢募集有关[J].浙江医学,2017,39(22):1957-1960,1980,5.基金项目
浙江省基础公益研究计划项目(LGF18H090018) （LGF18H090018）
绍兴市人民医院青年科研基金项目(2017A10) （2017A10）
