作物学报2018，Vol.44Issue(1)：15-23,9.DOI:10.3724/SP.J.1006.2018.00015
控制普通野生稻种子休眠性QTL的定位
Mapping of QTLs for Seed Dormancy in Oryza rufipogon Griff
摘要
Abstract
Seed dormancy of rice is an important agronomic trait related to rice quality and quantity. Studies on genetics and mo-lecular mechanisms of rice seed dormancy are of great significance in breeding fine rice varieties with moderate dormancy. In this research, a set of chromosome segment substitution lines (CSSLs), derived from an indica rice variety 9311 as the recurrent parent and the Oryza rufipogon Griff. as the donor parent, were used to detect the QTLs for dormancy of seeds at different storage dates after harvest. A total of 14 QTLs were detected on chromosomes 3, 4, 5, 6, 7, 10, 11, and 12. The lines with significantly stronger dormancy than the background parent 9311 were selected, showing the more dormancy loci in the lines the more strong dormancy. The F2 population of the cross between Q14 and 9311 was used to verify the QTLs for seed dormancy. A significant dormancy locus qSD-7-2 was mapped on chromosome 7 between the markers RM180 and RM21323, its LOD was 18.49 and the phenotypic variation rate was 33.53％. On this major stable inherited QTL, the allele gene from Oryza rufipogon Griff. significantly increased the dormancy of seeds. These results are available for map-based cloning of major QTLs for seed dormancy, and provide the breeding materials for cultivating appropriate dormant rice varieties.关键词
野生稻/种子休眠/染色体片段置换系/QTLKey words
Oryza rufipogon Griff./seed dormancy/CSSL/QTL引用本文复制引用
孙爱伶,伍洪铭,陈高明,张天雨,曹鹏辉,刘世家,江玲,万建民..控制普通野生稻种子休眠性QTL的定位[J].作物学报,2018,44(1):15-23,9.基金项目
本研究由国家重点研发计划项目(2016YFD0100101-08), 江苏省农业科技自主创新资金课题(CX[16]1029), 安徽省科技重大专项(16030701068)和江苏省现代作物生产协同创新中心项目资助.This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), the Agricultural Science and Technology Independent Innovation Fund Project of Jiangsu Province (CX[16]1029), the Science and Technology Major Project of Anhui Province (16030701068), and Jiangsu Collaborative Innovation Center for Modern Crop Production. （2016YFD0100101-08）
