作物学报2018，Vol.44Issue(1)：24-31,8.DOI:10.3724/SP.J.1006.2018.00024
水稻ABA生物合成基因OsNCED3响应干旱胁迫
ABA Biosynthesis Gene OsNCED3 Confers Drought Stress Tolerance in Rice
摘要
Abstract
NCED (9-cis-epoxycarotenoid dioxygenase), encoded by NCED gene family, is a rate limited enzyme responsible for the ABA biosynthesis in plants. It remains unknown whether OsNCED genes are responsible for controlling ABA levels during drought stress in rice. Among the five OsNCED genes, we found that OsNCED3 mRNA level was promptly induced by PEG-mimic drought stress and decreased by re-watering, with a tendency of well consistent with the variation of ABA content. Down-regulating of OsNCED3 gene expression in RNA interference (RNAi)-transgenic plants decreased the total biomass and showed a hypersensitive phenotype subjecting to drought stress, while the overexpression (OE)-transgenic seedlings increased the drought stress tolerance compared with the wild-type (WT). ABA contents in OsNCED3-OE leaves were higher than those in WT, meanwhile, OsNCED3-OE lines also increased the activities of anti-oxidative enzyme including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and expressions of stress/drought-related genes, i.e. dehydrin protein, LEA protein under drought stress. OsNCED3-RNAi lines showed an opposite tendency with the OsNCED3-OE plants. We therefore conclude that OsNCED3 gene plays an important role in controlling ABA level and drought stress resistance in rice.关键词
OsNCED3基因/干旱胁迫/脱落酸/水稻Key words
OsNCED3 gene/drought stress/abscisic acid/Oryza sativa引用本文复制引用
徐学中,汪婷,万旺,李思慧,朱国辉..水稻ABA生物合成基因OsNCED3响应干旱胁迫[J].作物学报,2018,44(1):24-31,8.基金项目
本研究由国家自然科学基金项目(31171466, 31570250)和国家重点基础研究发展计划(2012CB114306)资助.This study was supported by the National Natural Science Foundation of China (31171466) and the National Basic Research Program of China (2012CB114306). （31171466, 31570250）
