作物学报2018，Vol.44Issue(1)：32-42,11.DOI:10.3724/SP.J.1006.2018.00032
全基因组关联定位籼稻种质资源外观和加工品质QTL
Genome-wide Association Study of Grain Appearance and Milling Quality in a Worldwide Collection of Indica Rice Germplasm
摘要
Abstract
Appearance and milling quality are two crucial properties of rice affecting their market acceptability. Understanding the genetic basis of rice grain quality could improve the efficiency of breeding for high quality. Here we carried out a genome-wide association analysis using the 404k SNP genotype and data of grain appearance and milling quality for a diverse panel of 400 indica accessions selected from 3k Rice Genome Project collected in two environments. These were closely correlation between grain shape and quality (appearance and milling) of indica rice. The chalk trait reduced milled rice rate and high milled rice rate. In addition to grain shape, the phenotype value of appearance and milling quality traits was obviously affected by environments. Total of 39 QTL were detected which was significantly associated with grain appearance and milling quality in two environments and 17 of them were new QTL. Six new QTL (qMRR9, qGL2, qGL10, qGW1, qGLWR1, and qGLWR2.1) with minor or small effect were considered as stably expressed QTL because they were simultaneously identified in two environments. In addition, we found 21 QTL probably having multiple-effect in appearance and milling quality of indica rice. Combined with the results of pre-vious studies, we concluded that qSW5, GSE5, and GS3 play an important role in affecting appearance and milling quality. This research provides valuable information for cloning QTL controlling appearance and milling quality and breeding rice with high yield and good quality by molecular techniques.关键词
籼稻种质资源/外观与加工品质/全基因组关联分析/数量性状座位Key words
indica germplasms/appearance and milling quality/GWAS/quantitative trait loci/locus (QTL)引用本文复制引用
方雅洁,朱亚军,吴志超,陈凯,申聪聪,石英尧,徐建龙..全基因组关联定位籼稻种质资源外观和加工品质QTL[J].作物学报,2018,44(1):32-42,11.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2014AA10A601), 农业部引进国际先进农业科学技术(948)项目(2016-X16), 深圳孔雀团队计划(20130415095710361)和中国农业科学院科技创新工程团队项目资助.This study was supported by the National High-Tech Research & Development Program (863 Program) (2014AA10A601), the Introduction of International Advanced Agricultural Science and Technology of Ministry of Agriculture (948) (2016-X16), ShenZhen Peacock Plan (20130415095710361), and the Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences. （863计划）
