中国中西医结合杂志2017，Vol.37Issue(10)：1220-1225,6.DOI:10.7661/j.cjim.20170821.201
艾灸对慢性心力衰竭大鼠心功能及心肌组织TNF-α、NF-κB表达水平的影响
Effect of Moxibustion on Cardiac Function and Expression of TNF-α and NF-κB in Chronic Heart Failure Rats
摘要
Abstract
Objective To observe the effect of moxibustion on cardiac function and expression of TNF-α and NF-κB in chronic heart failure (CHF) rats,and to explore its mechanism underlying improvement of CHF.Methods The CHF model was established by intraperitoneal injection of Adriamycin.After successful modeling,28 rats were randomly divided into the model group,the moxibustion group,the Western medicine group,and the moxibustion +Western medicine group,7 in each group.Rats in the mild moxibustion group recieved mild moxibustion on bilateral Feishu (BL13) and Xinshu (BL15) for 15 min.Rats in the Western medicine group were administered by gastrogavage with Captopril (25 mg/kg),While those in the moxibustion + Western medicine group were administered with Captopril (25 mg/kg) and mild moxibustion.Once a day for 3 weeks.After treatment,heart failure(HR),cardiac output(CO),left ventricular systolic pressure (LVSP),left ventricular end-diastolic pressure (LVEDP),maximal rate of the decrease/increase of left ventricular pressure(±dp/dtmax) and the expression of NF-κB and TNF-α mRNA were observed respectively.Results In comparison with normal rats,characteristic behaviors and cardiac function were significantly different with modeling rats (P ＜0.01).The results of HE stain in myocardium were in accordance with the performance of CHF.Compared with the normal group,HR and LVEDP increased,CO,LVSP and ±dp/dtmax decreased (P ＜0.01),and the expression levels of NF-κB and TNF-α mRNA decreased significantly in the model group(P ＜0.01).Compared with the model group,CO,LVSP and ±dp/dtmax increased,HR and LVEDP were decreased and the expression levels of NF-κB and TNF-α mRNA were decreased significantly in the other three treatments groups(P ＜0.01).Compared with the moxibustion group and the Western medicine group,HR and LV-EDP decreased,CO,LVSP and ±dp/dtmax increased more obviously(P ＜0.05),and the level of TNF-αmRNA was decreased in the moxibustion + Western medicine group(P ＜0.05).Conclusions Moxibustion combined with medication could better improve the heart function and down-regulate the expression of TNF-α mRNA than using moxibustion or Western medicine alone,and could suppress CHF induced down-regulation of using expression of myocardial TNF-α mRNA and NF-κB in rats,which may contribute to its effect in regulating the immune response and relieving myocardial injury.关键词
艾灸/慢性心力衰竭/肿瘤坏死因子-α/核因子-κBKey words
moxibustion/chronic heart failure/tumor necrosis factor-α/nuclear transcription factor kappa B引用本文复制引用
贾学昭,李丹,王茎,曾永蕾,刘娜娜,杨佳辉..艾灸对慢性心力衰竭大鼠心功能及心肌组织TNF-α、NF-κB表达水平的影响[J].中国中西医结合杂志,2017,37(10):1220-1225,6.基金项目
国家自然基金面上资助项目(No.81574084,No.81273856) （No.81574084,No.81273856）
安徽省自然基金面上资助项目(No.1608085MH230) （No.1608085MH230）
安徽省高校科研创新平台建设项目(No.2015TD 033) （No.2015TD 033）
