安徽农业科学2018，Vol.46Issue(1)：1-4,23,5.
外源多胺促进的霍山石斛类原球茎芽发生与H2O2代谢的关系
Relationship between Enhanced Shoot Conversion and H2 O2-metabolism of Protocorm-like Bodies of Dendrobium huoshanense by Exogenous Polyamines
摘要
Abstract
[Objective] The research aimed to study the relationship between enhanced conversion of protocorm-like bodies (PLBs) of Dendrobium huoshanense to shoots by exogenous polyamines (PAs) and H2O2-metabolizing enzyme activities.[Method] We mainly measured the dynamic changes of H2O2 and the activities of metabolizing enzyme after exogenous polyamines treatment.[Result] Exogenous addition of PAs not only promoted the conversion frequency of PLBs to shoots but also elevated the levels of H2O2,compared with the control,the H2O2 levels in PAs groups increased by 175.6％ (Spd) and 180.6％ (Put) on the 5th day after being treated using 2.0 mmol/L Spd or Put for 5 days.Dimethylthiourea (DMTU),the scavenger of H2O2,inhibited conversion of PLBs to shoots and the effect could be offset partly by exogenous PAs.Although the activities of superoxide dismutase (SOD) and peroxidase (POD) did not change significantly in the early and middle stages of exogenous polyamine treatment,the activity of polyamine oxidase (PAO) for synthesizing H2O2,ascorbate peroxidase (APX) for dissolving H2O2 and catalase (CAT) were significantly increased.[Conclusion]The occurrence of protocorm buds of Dendrobium huoshanense with the action of exogenous polyamines is related to the accumulation and catabolism of H2O2.关键词
霍山石斛/外源多胺/类原球茎/芽发生/代谢酶Key words
Dendrobium huoshanense/Exogenous polyamines/Protocorm-like bodies/Shoot morphogenesis/Metabolizing enzymes分类
农业科技引用本文复制引用
杨光,罗建平,王瑛..外源多胺促进的霍山石斛类原球茎芽发生与H2O2代谢的关系[J].安徽农业科学,2018,46(1):1-4,23,5.基金项目
河北大学自然科学研究计划项目(2010-195). （2010-195）
