安徽农业科学2018，Vol.46Issue(1)：105-107,3.
红蛇果接穗组织培养体系的建立
Establishment of Tissue Culture System of Red Delicious Scion
胡春宏 1王婷婷 1常苹 1季翔1
作者信息
- 1. 江苏东郁园林科技有限公司,江苏无锡214000
- 折叠
摘要
Abstract
[Objective] To establish the tissue culture system of red delicious.[Method] Semi-woody stem segments from two-years red delicious seedlings were chosen as the explants in the early spring to establish the tissue culture system by sterilization,germination,multiplication and rooting process.[Result]The most suitable germination medium was MS +6-BA (1.0 mg/L) + IBA (0.1 mg/L) and the sprout rate can up to 93.33％.Adding PVP (0.5 g/L) and Vc (100 mg/L) can effectively prevent browning.The germinated buds were transferred into MS +6-BA (1.2 ～ 1.6 mg/L) which could bring a highly efficient multiplication and inhibit the vitrification with a multiplication rate of 3.79.The most suitable rooting medium for the apical buds was 1/2MS + IBA(1.0 mg/L) and the rooting rate was 34.17％.[Conclusion] The study provided a technical basis for providing a large number of tissue culture seedling of red delicious and subsequent micrografts.关键词
接穗/红蛇果/组织培养Key words
Scion/Red delicious/Tissue culture分类
农业科技引用本文复制引用
胡春宏,王婷婷,常苹,季翔..红蛇果接穗组织培养体系的建立[J].安徽农业科学,2018,46(1):105-107,3.
