北华大学学报（自然科学版）2018，Vol.19Issue(1)：49-52,4.DOI:10.11713/j.issn.1009-4822.2018.01.009
基于PCR-RFLP的西洋参和人参指纹特征鉴定
Fingerprint Dentification of Panax Quinquefolius and Panax ginseng Based on PCR-RFLP
摘要
Abstract
Objective To establish DNA PCR-RFLP method for the identification of Panax quinquefolius and Panax ginseng. Method The modified CTAB method was used to extract genome DNA from Panax quinquefolius and Panax ginseng. Specific primers were designed with bioinformatics software of ribosomal DNA ITS sequences for Panax quinquefolius and Panax ginseng. The specified sequence was amplified by PCR technology,and the enzyme products obtained by the method of RFLP were identified and compared for fingerprint. Results DNA 19 kb genome fragments were obtained from Panax quinquefolius and Panax ginseng. The 122 bp size of the ribosomal gene fragment was amplified. After enzyme digestion,Panax quinquefolius had two fragments of 80 bp and 42 bp,while Panax ginseng had only a single fragment of 122 bp. Conclusion Both the DNA fingerprints of Panax quinquefolius and Panax ginseng had specific enzyme site which could be used as an effective method for identification. The experimental results showed that the method was simple,rapid and reliable.关键词
西洋参/人参/PCR-RFLP方法/DNA酶切Key words
Panax quinquefolius/Panax ginseng/PCR-RFLP method/DNA enzyme digestion分类
医药卫生引用本文复制引用
王雪松,周雨晴,李丹,夏薇,李明,李明成..基于PCR-RFLP的西洋参和人参指纹特征鉴定[J].北华大学学报（自然科学版）,2018,19(1):49-52,4.基金项目
吉林省教育厅科学技术研究项目(2012133) （2012133）
吉林市科技计划项目(2013523010 （）
2015420013) （）
吉林省战略性新兴产业和高新技术产业发展项目(2013G030). （2013G030）
