重庆医学2018，Vol.47Issue(1)：4-6,10,4.DOI:10.3969/j.issn.1671-8348.2018.01.002
大黄灵仙胶囊调控ABCB11和ABCC2干预胆结石形成的作用机制研究
Study on mechanism of Dahuanglingxian Capsule for intervening gallstone formation by regulating ABCB11 and ABCC2
摘要
Abstract
Objective To explore the mechanism of Dahuanglinxian Capsule for intervening gallstone formation by regulating the expression levels of ABCB11 and ABCC2 mRNA and protein.Methods Forty male C57BL/6 mice were divided into the normal group(group N),model group (group M),ursodeoxycholic acid group (group U) and Dahuanglinxian Capsule treatment group (group D),10 cases in each group.The group N was fed with normal diet,while the group M,U and D were fed with lithogenic fodder for 8 weeks.Meanwhile the group U and D were given the medication intervention,once daily,for continuous 8 weeks of gavage.After successful modeling,mRNA and protein expression levels of ABCB11 and ABCC2 were detected by RT-PCR and immunohistochemistry.Results Compared with the other three groups,the expressions of ABCB11 and ABCC2 mRNA gene and protein in M group were significantly reduced(P＜0.01);while,there was no statistical difference in the expressions of ABCB11 and ABCC2 mRNA and protein between the group D and N(P＞0.05).Conclusion Dahuanglinxian Capsule can prevent the gallstone formation by regulating the expression of ABCB11 and ABCC2 mRNA and protein.关键词
胆结石/大黄灵仙胶囊/ABCB11/ABCC2Key words
gallstone/Dahuanglinxian Capsules/ABCB11/ABCC2分类
医药卫生引用本文复制引用
唐乾利,吕震,王兵,王宇,王澍,舒清峰,葛斌,谢思圳..大黄灵仙胶囊调控ABCB11和ABCC2干预胆结石形成的作用机制研究[J].重庆医学,2018,47(1):4-6,10,4.基金项目
国家自然科学基金面上项目(81470198) （81470198）
广西科学研究与技术开发计划项目(桂科2017AB45053). （桂科2017AB45053）
