重庆医学2018，Vol.47Issue(1)：17-19,3.DOI:10.3969/j.issn.1671-8348.2018.01.006
死亡结构域相关蛋白对卵巢癌细胞周期及化疗影响的研究
Study on influence of Daxx on cell cycle and chemotherapy in human ovarian cancer cells
伦巍巍 1王俊耐1
作者信息
- 1. 郑州大学第三附属医院妇产科 450015
- 折叠
摘要
Abstract
Objective To explore the influence of down-regulating Daxx on cell cycle and chemotherapeutic drug resistance in human ovarian cancer cells.Methods SiRNA and NC negative control(NC) RNA of Daxx were constructed and divided into the NC group and silencing Daxx(siDaxx) group after transfecting to ovarian cancer cell line C13k.The doxorubicin concentration gradient of 0,0.15,0.30,0.60 μmol/L was set.The cellular cycle changes and apoptosis changes of these two groups were detected by using the flow cytometry.Western blot was used to detect the expression changes of apoptosis related protein cyclinB1 and cleavedparp.Results 0.30 μmol/L doxorubicin down-regulated Daxx to result in significant G2/M arrest(P＜0.05).Down-regulating Daxx resulted in doxorubicin resistance in C13k cells.Conclusion The effect of Daxx on ovarian cancer chemotherapy might be related to its regulation on cell cycle.关键词
Fas相关死亡结构域蛋白质/卵巢肿瘤/细胞周期/cyclinB1Key words
Fas-associated death domain protein/ovarian neoplasms/cell cycle/cyclineB1分类
医药卫生引用本文复制引用
伦巍巍,王俊耐..死亡结构域相关蛋白对卵巢癌细胞周期及化疗影响的研究[J].重庆医学,2018,47(1):17-19,3.
