重庆医学2018，Vol.47Issue(1)：20-22,3.DOI:10.3969/j.issn.1671-8348.2018.01.007
穿龙薯蓣皂苷元对小鼠T淋巴细胞影响的体外研究
In vitro study on effects of diosgenin from Dioscorea nipponica on mouse T lymphocytes
王烨 1付聪敏 1贾丽荣 1李松燃 2党晓伟 1马秀芬 1秦博文1
作者信息
- 1. 承德护理职业学院,河北承德067000
- 2. 空军军医大学,陕西西安710032
- 折叠
摘要
Abstract
Objective To study the effect of diosgenin(Dio) from Dioscorea nipponica on the proliferation in mouse spleen T lymphocytes and expressions of IL-2 and IFN-γmRNA,in order to investigate the immunity regulatory mechanism of Dio.Methods T lymphocytes stimulated by different concentrations of Dio and concanavalin A(ConA) were co-cultured.CCK-8 was used to detect the Dio effects on T lymphocyte proliferation.The RT-PCR method was adopted to detect the effect of Dio on expression of IL-2 and IFN-γmRNA.Results The Dio concentration in the range of 0.937 5-15.000 0 μg/mL had the inhibiting effect on T lymphocyte proliferation,Dio concentration in the range of 3.750 0-15.000 0μg/mL had the inhibiting effect on IFN-γ and IL-2 expression in T lymphocytes.With the Dio concentration increase,the inhibition effect was enhanced(P＜0.05).7.500 0 μg/mL was the best inhibition concentration.The inhibition effect was decreased when the concentration exceeding 7.500 0 μg/mL.Conclusion Dio has the inhibiting effect on T lymphocyte proliferation and expressions of IL-2 and IFN-γ mRNA.关键词
穿龙薯蓣皂苷元/T淋巴细胞/白细胞介素-2/干扰素Ⅱ型/mRNAKey words
diosgenin/T lymphocyte/interleukin-2/interferon type Ⅱ/mRNA分类
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王烨,付聪敏,贾丽荣,李松燃,党晓伟,马秀芬,秦博文..穿龙薯蓣皂苷元对小鼠T淋巴细胞影响的体外研究[J].重庆医学,2018,47(1):20-22,3.
