儿科药学杂志2018，Vol.24Issue(1)：1-4,4.DOI:10.13407/j.cnki.jpp.1672-108X.2018.01.001
初发过敏性紫癜和紫癜性肾炎患儿滤泡辅助性T细胞变化初探
Changs of T Follicular Helper Cell in Children Primary Henoch-Sch(o)nlein Purpura and Henoch-Sch(o)nlein Purpura Nephrits
摘要
Abstract
Objective:To explore the changes and mechanism of T follicular helper (Tfh) cells in children with primary HenochSch(o)nlein purpura (HSP) and Henoch-Sch(o)nlein purpura nephritis (HSPN).Methods:Forty-five children with HSP were enrolled in this study.The proportion of CD4+ CXCR5+ ICOS+T cells in peripheral blood was analyzed by flow cytometry.Real-time PCR was performed to detect the level of Tfh cells transcriptional factor (Bcl-6) and its inhibitor (Blimp-1).The plasma concentrations of IL-21 and IL-4 were determined by ELISA.HSP children were followed up for 6 months,depending on whether the kidney was involved,they were divided into HSP group (26 cases) and HSPN group (19 cases).Twenty health children were selected as the control group.Results:(1) The proportion of Tfh cells in HSP group was significantly higher than control group (3.75％ ± 1.32％ vs 2.01％ ± 0.72％,P＜0.05).The proportion of Tfh cells in HSPN group was significantly higher than that of HSP group (5.86％ ± 1.94％ vs 3.75％ ±1.32％,P＜0.05).(2) The mRNA expression of Bcl-6 was significantly elevated in HSPN group,which was significantly higher than that of HSP group (P＜0.05),and both of them were higher than that of control group (P＜0.05).Simultaneously,the mRNA expression of Blimp-1 was found to be down-regulated in HSPN group,which was significantly lower than that of HSP group (P＜ 0.05),and both of them were lower than control group (P＜0.05).(3) The plasma IL-21 concentration in HSPN group was significantly higher than that of HSP group (P＜0.05),and both of them were significantly increased in comparison with control group (P＜0.05).There was no significantly difference of the plasma IL-4 concentration between HSP group and HSPN group (P＞0.05),but both of them were higher than that of control group (P＜0.05).Conclusion:The over-activation of Tfh cells may be correlated with immune pathogenesis in HSP.Aberrant activation of Tfh cells may be one of factors causing disturbed HSPN.关键词
滤泡辅助性T细胞/Bcl-6/Blimp-1/过敏性紫癜/肾炎Key words
T follicular helper cell/Bcl-6/Blimp-1/Henoch-Sch(o)nlein purpura/nephritis分类
医药卫生引用本文复制引用
贾实磊,杨军,马颐姣,李薇玢,李成荣,周厚福..初发过敏性紫癜和紫癜性肾炎患儿滤泡辅助性T细胞变化初探[J].儿科药学杂志,2018,24(1):1-4,4.基金项目
深圳市医学科技项目基金资助,编号201607048. （）
