中国妇幼健康研究2017，Vol.28Issue(12)：1593-1596,4.DOI:10.3969/j.issn.1673-5293.2017.12.029
沉默干细胞转录因子Sox2对宫颈癌细胞增殖能力影响的研究
Effect of silencing stem cell transcription factor Sox 2 on proliferation of cervical cancer cells
摘要
Abstract
Objective To study the influence of silencing stem cell transcription factor Sox 2 on cervical cancer cell proliferation and its mechanism .Methods Interference plasmid (shRNA-Sox2) was stably transfected into cervical cancer cell line C33A ,then C33A clonal cell line with stably silenced Sox2-expression (shRNA-Sox2-C33A ) was screened by puromycin selection .M TT assay and colony-forming experiment were used to detect effect of silencing Sox 2 expression on cervical cancer cell proliferation ability . Change of cell cycle was studied by flow cytometry (FCM ) ,while expression of cell cycle related protein CyclinD1 was detected by Western blot .Results The mRNA expression of Sox2 was down-regulated in C33A cells after shRNA-Sox2 transfection (t = 7 .94 ,P < 0 .01) .Proliferation rate of shRNA-Sox2-C33A group was low (t = 10 .93 ,P < 0 .05) .Down regulation of Sox2 expression also led to decreased cloning formation ability of cervical cancer cells in vitro (t = 8 .30 , P < 0 .01) .Proportion of cells in G0/G1 phase was increased ,while proportion of cells in S phase was reduced (t value was - 4 .60 and 4 .67 ,respectively ,both P < 0 .05) . Decreased expression of CyclinD1 was detected by Western blot (t = 113 .79 , P < 0 .05) .Conclusion Silencing of Sox2 expression might stop cell from entering into S phase from G1 phase through down regulation of CyclinD1 ,inhibiting proliferation of cervical cancer cells .关键词
Sox2基因/宫颈癌/基因沉默/细胞增殖Key words
Sox2 gene/cervcial cancer/silencing of gene/cell proliferation分类
医药卫生引用本文复制引用
冀静,左萍,黄康榕,刘海娟,方静,王月玲..沉默干细胞转录因子Sox2对宫颈癌细胞增殖能力影响的研究[J].中国妇幼健康研究,2017,28(12):1593-1596,4.基金项目
国家科学青年基金资助项目(81001162) （81001162）
陕西省自然科学基金重点研发计划资助项目(2017SF-015) （2017SF-015）
西安交通大学第一附属医院院基金资助项目(YK201503) （YK201503）
